Glucose-Regulated Protein 78 (GRP78) Mediated the Efficacy to Curcumin Treatment on Hepatocellular Carcinoma

被引：35

作者：

Chang, Yu-Jia ^{[1
,2
,3
,4
,5
]}

Tai, Cheng-Jeng ^{[6
]}

Kuo, Li-Jen ^{[1
,2
,3
,4
]}

Wei, Po-Li ^{[2
,3
,4
,5
]}

Liang, Hung-Hua ^{[2
,3
]}

Liu, Tsan-Zon ^{[9
]}

Wang, Weu ^{[2
,3
]}

Tai, Chen-Jei ^{[10
,11
]}

Ho, Yuan-Soon ^{[1
,5
,7
]}

Wu, Chih-Hsiung ^{[1
,2
,3
,8
]}

Huang, Ming-Te ^{[1
,2
,3
,8
]}

机构：

[1] Taipei Med Univ, Coll Med, Grad Inst Med Sci, Taipei, Taiwan

[2] Taipei Med Univ, Coll Med, Sch Med, Dept Surg, Taipei, Taiwan

[3] Taipei Med Univ, Taipei Med Univ Hosp, Dept Surg, Div Gen Surg, Taipei, Taiwan

[4] Taipei Med Univ, Taipei Med Univ Hosp, Ctr Canc, Taipei, Taiwan

[5] Taipei Med Univ, Ctr Excellence Canc Res, Taipei, Taiwan

[6] Taipei Med Univ & Hosp, Dept Med, Div Hematol & Oncol, Taipei, Taiwan

[7] Taipei Med Univ, Coll Med Sci & Technol, Sch Med Lab Sci & Biotechnol, Taipei, Taiwan

[8] Taipei Med Univ, Shuang Ho Hosp, Dept Surg, Div Gen Surg, Taipei, Taiwan

[9] Taipei Med Univ Hosp, Ctr Canc, Translat Res Lab, Taipei, Taiwan

[10] Taipei Med Univ, Sch Med, Dept Obstet & Gynecol, Taipei, Taiwan

[11] Taipei Med Univ Hosp, Dept Chinese Med, Taipei, Taiwan

来源：

ANNALS OF SURGICAL ONCOLOGY | 2011年 / 18卷 / 08期

关键词：

COLON-CANCER CELLS; SORAFENIB; APOPTOSIS; THERAPY; FRAGMENTATION; ANGIOGENESIS; MANAGEMENT; RESISTANCE; SURVIVAL; PATHWAY;

D O I：

10.1245/s10434-011-1597-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells.

引用

页码：2395 / 2403

页数：9

共 37 条

[1] Molecular targets of dietary agents for prevention and therapy of cancer [J].

Aggarwal, BB ;

Shishodia, S .

BIOCHEMICAL PHARMACOLOGY, 2006, 71 (10) :1397-1421

[2] Pharmacological basis for the role of curcumin in chronic diseases: an age-old spice with modern targets [J].

Aggarwal, Bharat B. ;

Sung, Bokyung .

TRENDS IN PHARMACOLOGICAL SCIENCES, 2009, 30 (02) :85-94

[3] Curcumin and cancer: An "old-age" disease with an "age-old" solution [J].

Anand, Preetha ;

Sundaram, Chitra ;

Jhurani, Sonia ;

Kunnumakkara, Ajaikumar B. ;

Aggarwal, Bharat B. .

CANCER LETTERS, 2008, 267 (01) :133-164

[4] Review article: the management of hepatocellular carcinoma [J].

Cabrera, R. ;

Nelson, D. R. .

ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2010, 31 (04) :461-476

[5] Glucose-Regulated Protein 78 (GRP78) Silencing Enhances Cell Migration but Does Not Influence Cell Proliferation in Hepatocellular Carcinoma [J].

Chang, Yu-Jia ;

Chiu, Chong-Chi ;

Wu, Chih-Hsiung ;

An, Jane ;

Wu, Cheng-Chia ;

Liu, Tsan-Zon ;

Wei, Po-Li ;

Huang, Ming-Te .

ANNALS OF SURGICAL ONCOLOGY, 2010, 17 (06) :1703-1709

[6] Efficacy and safety of sorafenib in patients in the Asia-Pacific region with advanced hepatocellular carcinoma: a phase III randomised, double-blind, placebo-controlled trial [J].

Cheng, Ann-Lii ;

Kang, Yoon-Koo ;

Chen, Zhendong ;

Tsao, Chao-Jung ;

Qin, Shukui ;

Kim, Jun Suk ;

Luo, Rongcheng ;

Feng, Jifeng ;

Ye, Shenglong ;

Yang, Tsai-Sheng ;

Xu, Jianming ;

Sun, Yan ;

Liang, Houjie ;

Liu, Jiwei ;

Wang, Jiejun ;

Tak, Won Young ;

Pan, Hongming ;

Burock, Karin ;

Zou, Jessie ;

Voliotis, Dimitris ;

Guan, Zhongzhen .

LANCET ONCOLOGY, 2009, 10 (01) :25-34

[7] Glucose-Regulated Protein 78 Is a Novel Contributor to Acquisition of Resistance to Sorafenib in Hepatocellular Carcinoma [J].

Chiou, Jeng-Fong ;

Tai, Cheng-Jeng ;

Huang, Ming-Te ;

Wei, Po-Li ;

Wang, Yu-Huei ;

An, Jane ;

Wu, Chih-Hsiung ;

Liu, Tsan-Zon ;

Chang, Yu-Jia .

ANNALS OF SURGICAL ONCOLOGY, 2010, 17 (02) :603-612

[8] Curcumin suppresses growth and chemoresistance of human glioblastoma cells via AP-1 and NFκB transcription factors [J].

Dhandapani, Krishnan M. ;

Mahesh, Virendra B. ;

Brann, Darrell W. .

JOURNAL OF NEUROCHEMISTRY, 2007, 102 (02) :522-538

[9]

Hsu CH, 2007, ADV EXP MED BIOL, V595, P471

[10] Geographic difference in survival outcome for advanced hepatocellular carcinoma: Implications on future clinical trial design [J].

Hsu, Chiun ;

Shen, Ying-Chun ;

Cheng, Chia-Chi ;

Hu, Fu-Chang ;

Cheng, Ann-Lii .

CONTEMPORARY CLINICAL TRIALS, 2010, 31 (01) :55-61

← 1 2 3 4 →