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Mitochondrial depolarization accompanies cytochrome c release during apoptosis in PC6 cells

被引：304
作者：
Heiskanen, KM
Bhat, MB
Wang, HW
Ma, JJ
Nieminen, AL
机构：
[1] Case Western Reserve Univ, Sch Med, Dept Anat, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Sch Med, Dept Physiol & Biophys, Cleveland, OH 44106 USA
[3] Case Western Reserve Univ, Sch Med, Dept Biomed Engn, Cleveland, OH 44106 USA
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1999年 / 274卷 / 09期
关键词：
D O I：
10.1074/jbc.274.9.5654
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cytochrome c is released from mitochondria into the cytosol in cells undergoing apoptosis. The temporal relationship between cytochrome c release and loss of mitochondrial membrane potential was monitored by laser-scanning confocal microscopy in single living pheochromocytoma-6 cells undergoing apoptosis induced by staurosporine. Mitochondrial membrane potential monitored by tetramethylrhodamine methyl ester decreased abruptly in individual cells from 2 to 7 h after treatment with staurosporine. Depolarization was accompanied by cytochrome c release documented by release of transfected green fluorescent protein-tagged cytochrome c in these cells. The results show that mitochondrial depolarization accompanies cytochrome c release in pheochromocytoma-6 cells undergoing apoptosis.
引用
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页码：5654 / 5658
页数：5
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