A dialysis system with phytoplankton for monitoring chemical pollution in freshwater ecosystems by alkaline phosphatase assay

被引：15

作者：

Durrieu, C ^{[1
]}

Badreddine, I ^{[1
]}

Daix, C ^{[1
]}

机构：

[1] Ecole Natl Travaux Publ Etat, Lab Sci Environm, F-69518 Vaulx En Velin, France

来源：

JOURNAL OF APPLIED PHYCOLOGY | 2003年 / 15卷 / 04期

关键词：

assay; dialysis; heavy metals; pesticides; phenol compounds; phosphatase; phosphomonoesterase; phytoplankton; toxicity; water pollution;

D O I：

10.1023/A:1025165727616

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of three groups of chemicals, 'heavy' metals, pesticides and phenolics, on alkaline phosphatase activity of intact Chlorella vulgaris cells were investigated. There was a marked inhibitory effect of heavy metals and a slight one due to phenolics, but the pesticides tested showed no effect. In order to detect heavy metals in freshwater ecosystems, we propose a dialysis system, which can be placed in the field and then provide early warning signals of toxicity. This phosphatase inhibition test is recommended the first stage of an enzymatic screening system for chemical pollution in water.

引用

页码：289 / 295

页数：7

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