Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments

被引：238

作者：

Lipfert, Jan ^{[1
]}

Kerssemakers, Jacob W. J. ^{[1
]}

Jager, Tessa ^{[1
]}

Dekker, Nynke H. ^{[1
]}

机构：

[1] Delft Univ Technol, Fac Sci Appl, Kavli Inst Nanosci, Dept Bionanosci, Delft, Netherlands

来源：

NATURE METHODS | 2010年 / 7卷 / 12期

关键词：

OPTICAL TWEEZERS; SUPERCOILED DNA; MOLECULE; ELASTICITY; RIGIDITY; IB;

D O I：

10.1038/NMETH.1520

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce magnetic torque tweezers, which enable direct single-molecule measurements of torque. Our measurements of the effective torsional stiffness C of dsDNA indicated a substantial force dependence, with C = similar to 40 nm at low forces up to C = similar to 100 nm at high forces. The initial torsional stiffness of RecA filaments was nearly twofold larger than that for dsDNA, yet at moderate torques further build-up of torsional strain was prevented.

引用

页码：977 / U54

页数：6

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