Magnetic Tweezers Measurement of Single Molecule Torque

被引：95

作者：

Celedon, Alfredo ^{[1
,5
,6
,8
]}

Nodelman, Ilana M. ^{[2
]}

Wildt, Bridget ^{[4
]}

Dewan, Rohit ^{[3
]}

Searson, Peter ^{[4
]}

Wirtz, Denis ^{[3
,5
,6
]}

Bowman, Gregory D. ^{[2
,5
,6
]}

Sun, Sean X. ^{[1
,5
,6
,7
]}

机构：

[1] Johns Hopkins Univ, Dept Mech Engn, Baltimore, MD 21218 USA

[2] Johns Hopkins Univ, Dept Biophys, Baltimore, MD 21218 USA

[3] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA

[4] Johns Hopkins Univ, Dept Mat Sci & Engn, Baltimore, MD 21218 USA

[5] Johns Hopkins Univ, Howard Hughes Med Inst, Grad Training Program, Baltimore, MD 21218 USA

[6] Johns Hopkins Univ, Inst NanobioTechnol, Baltimore, MD 21218 USA

[7] Johns Hopkins Univ, Whitaker Biomed Engn Inst, Baltimore, MD 21218 USA

[8] Pontificia Univ Catolica Chile, Dept Ingn Mecan & Met, Santiago, Chile

来源：

NANO LETTERS | 2009年 / 9卷 / 04期

关键词：

CHROMATIN FIBERS; RNA-POLYMERASE; DNA; MICROMANIPULATION; ELASTICITY;

D O I：

10.1021/nl900631w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Torsional stress in linear biopolymers such as DNA and chromatin has important consequences for nanoscale biological processes. We have developed a new method to directly measure torque on single molecules. Using a cylindrical magnet, we manipulate a novel probe consisting of a nanorod with a 0.1 mu m ferromagnetic segment coupled to a magnetic bead. We achieve controlled introduction of turns into the molecule and precise measurement of torque and molecule extension as a function of the number of turns at low pulling force. We show torque measurement of single DNA molecules and demonstrate for the first time measurements of single chromatin fibers.

引用

页码：1720 / 1725

页数：6

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