New evidence for nitrogen fixation within the Italian white truffle Tuber magnatum

被引：81

作者：

Barbieri, Elena ^{[1
]}

Ceccaroli, Paola ^{[1
]}

Saltarelli, Roberta ^{[1
]}

Guidi, Chiara ^{[1
]}

Potenza, Lucia ^{[1
]}

Basaglia, Marina ^{[2
]}

Fontana, Federico ^{[2
]}

Baldan, Enrico ^{[2
]}

Casella, Sergio ^{[2
]}

Ryahi, Ouafae ^{[3
]}

Zambonelli, Alessandra ^{[3
]}

Stocchi, Vilberto ^{[1
]}

机构：

[1] Univ Urbino Carlo Bo, Dipartimento Sci Biomol, I-61029 Urbino, PU, Italy

[2] Univ Padua, Dipartimento Biotecnol Agr, Legnaro, Italy

[3] Univ Bologna, Dipartimento Protez & Valorizzaz Agroalimentare, Bologna, Italy

来源：

FUNGAL BIOLOGY | 2010年 / 114卷 / 11-12期

关键词：

Nitrogen-fixing bacteria; Truffle; Tuber magnatum; BACTERIAL DIVERSITY; GENE-SEQUENCES; NIFH GENES; EXPRESSION; SOIL; ECTOMYCORRHIZAS; IDENTIFICATION; COMMUNITIES; REDUCTION; STRAIN;

D O I：

10.1016/j.funbio.2010.09.001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Diversity of nitrogen-fixing bacteria and the nitrogen-fixation activity was investigated in Tuber magnatum, the most well-known prized species of Italian white truffle. Degenerate PCR primers were applied to amplify the nitrogenase gene nifH from T. magnatum ascomata at different stages of maturation. Putative amino acid sequences revealed mainly the presence of Alphaproteobacteria belonging to Bradyrhizobium spp. and expression of nifH genes from Bradyrhizobia was detected. The nitrogenase activity evaluated by acetylene reduction assay was 0.5-7.5 mu mol C(2)H(4)h(-1)g(-1), comparable with early nodules of legumes associated with specific nitrogen-fixing bacteria. This is the first demonstration of nitrogenase expression gene and activity within truffle. (C) 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

引用

页码：936 / 942

页数：7

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