Studies on neurosteroids VII. Determination of pregnenolone and its 3-stearate in rat brains using high-performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry

被引：29

作者：

Shimada, K ^{[1
]}

Mukai, Y ^{[1
]}

机构：

[1] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 714卷 / 02期

关键词：

steroids; neurosteroids; pregnenolone; pregnenolone-3-stearate;

D O I：

10.1016/S0378-4347(98)00227-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay method for pregnenolone and its 3-stearate in rat brains has been developed using LC-atmospheric pressure chemical ionization (APCI) isotope dilution MS. The extraction of the rat brain homogenate containing deuterated internal standards (ISs) with organic solvent followed by silica gel mini-column chromatography gave two fractions containing pregnenolone and its 3-stearate together with the respective IS. Each fraction was derivatized into 3-acetate-20-methyloxime and 20-methyloxime, respectively, followed by silica gel mini-column chromatography to remove the excess reagents, and then each derivatized fraction was applied onto reversed-phase LC-APCI-MS. The method was applied to the determination of these steroids in the gray matter and olfactory bulbs of rat brains, which were divided into control and acute stressed specimens. Although pregnenolone in both regions of the rat brains increased more than five times after stress, its 3-stearate decreased after stress. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：153 / 160

页数：8

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