Studies on neurosteroids. VII. Characterization of pregnenolone, its sulfate and dehydroepiandrosterone in rat brains using liquid chromatography mass spectrometry

被引：25

作者：

Shimada, K ^{[1
]}

Mukai, Y ^{[1
]}

Yago, K ^{[1
]}

机构：

[1] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 920, Japan

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1998年 / 21卷 / 06期

关键词：

D O I：

10.1080/10826079808000507

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation and characterization of pregnenolone, its 3-sulfate and dehydroepiandrosterone in rat brains (neurosteroids) were carried out using liquid chromatography/mass spectrometry. The genins obtained from rat brains were acetylated and then derivatized with O-methylhydroxylamine to give the respective acetate-methyloximes, which were identified by comparison of their chromatographic behavior with authentic samples during liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry. Pregnenolone 3-sulfate in rat brains was also identified with an authentic sample using liquid chromatography/electrospray ionization-mass spectrometry.

引用

页码：765 / 775

页数：11

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