The effects of phenobarbital and ketoconazole on the alkaloid biosynthesis in Catharanthus roseus cell suspension cultures

被引：18

作者：

Contin, A ^{[1
]}

Collu, G ^{[1
]}

van der Heijden, R ^{[1
]}

Verpoorte, R ^{[1
]}

机构：

[1] Gorlaeus Labs, Leiden Amsterdam Ctr Drug Res, Div Pharmacognosy, NL-2300 RA Leiden, Netherlands

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 1999年 / 37卷 / 02期

关键词：

Catharanthus roseus; cytochrome P450; geraniol; 10-hydroxylase; iridoids; ketoconazole; phenobarbital;

D O I：

10.1016/S0981-9428(99)80075-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The cytochrome P450 enzyme geraniol 10-hydroxylase plays an important role in the biosynthesis of terpenoid indole alkaloids in suspension cultures of Catharanthus roseus. The activity of this enzyme was induced by the treatment of cells with phenobarbital, and inhibited by treatment with ketoconazole. The alkaloid accumulation increased after phenobarbital treatment whereas it decreased after ketoconazole treatment. Phenobarbital and ketoconazole did not affect the in vivo conversion rate of loganin to secologanin, a reaction proposed to be catalyzed by a cytochrome P450 enzyme. (C) Elsevier, Paris.

引用

页码：139 / 144

页数：6

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