Coupling of mitosis to the completion of S phase through Cdc34-mediated degradation of Wee 1

被引：127

作者：

Michael, WM ^{[1
]}

Newport, J ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA

来源：

SCIENCE | 1998年 / 282卷 / 5395期

关键词：

D O I：

10.1126/science.282.5395.1886

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The dependence of mitosis on the completion of the period of DNA replication in the cell cycle [synthesis (S) phase] ensures that chromosome segregation occurs only after the genome has been fully duplicated. A key negative regulator of mitosis, the protein kinase Wee1, was degraded in a Cdc34-dependent fashion in Xenopus egg extra cts. Th is proteolysis event was required for a timely entrance into mitosis and was inhibited when DNA replication was blocked. Therefore, the DNA replication checkpoint can prevent mitosis by suppressing the proteolysis of Wee1 during 5 phase.

引用

页码：1886 / 1889

页数：4

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