Protein crystallography microdiffraction

被引：95

作者：

Riekel, C

Burghammer, M

Schertler, G

机构：

[1] European Synchrotron Radiat Facil, F-38043 Grenoble, France

[2] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2005年 / 15卷 / 05期

关键词：

D O I：

10.1016/j.sbi.2005.08.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein microdiffraction using monochromatic beams is becoming a routine tool at third-generation synchrotron radiation sources. Beam sizes have reached the scale of about 5 mu m, with illuminated crystal volumes of approximately 500 mu m(3), as shown for the case of bovine rhodopsin, which was refined to a resolution of 2.6 angstrom. Progress in X-ray optical systems and instrumentation will enable the method to be extended to smaller beams and smaller crystal volumes.

引用

收藏

页码：556 / 562

页数：7

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