Chiral separation of amino acids in ultrafiltration through DNA-immobilized cellulose membranes

被引：42

作者：

Higuchi, A ^{[1
]}

Hayashi, A

Kanda, N

Sanui, K

Kitamura, H

机构：

[1] Seikei Univ, Dept Appl Chem, Musashino, Tokyo, Japan

[2] Sophia Univ, Dept Chem, Chiyoda Ku, Tokyo 102, Japan

来源：

JOURNAL OF MOLECULAR STRUCTURE | 2005年 / 739卷 / 1-3期

基金：

以色列科学基金会;

关键词：

chiral separation; membrane; DNA; ultrafiltration;

D O I：

10.1016/j.molstruc.2004.05.040

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Ultrafiltration experiments for the chiral separation of racemic tryptophan, phenylglycine and phenylalanine were investigated through immobilized DNA membranes having various pore sizes. L-tryptophan preferentially permeated through immobilized DNA membranes with a pore size < 2.0 nm (molecular weight cut-off (MWCO) < 5000) while D-tryptophan preferentially permeated through immobilized DNA membranes with a pore size > 2.0 nm (MWCO > 5000). These results are completely opposite tendency in the ultrafiltration of racemic phenylalanine through the immobilized DNA membranes. This may be originated from the different interaction between DNA and tryptophan compared to that between DNA and phenylalanine. However, in both cases the pore size of the immobilized DNA membranes regulated preferential permeation of the enantiomer through the membranes. The immobilized DNA membranes are categorized as channel type membranes and not as affinity membranes. Chiral separation models were proposed from using the chiral separation results of racemic amino acids, preferential adsorption of amino acid enantiomers and EPMA results. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：145 / 152

页数：8

共 38 条

[1] Enantioselective permeation of racemates through a solid (+)-poly{2-[dimethyl(10-pinanyl)silyl]norbornadiene} membrane [J].