Characterization and early detection of grapevine (Vitis vinifera) stress responses to esca disease by in situ chlorophyll fluorescence and comparison with drought stress

被引:208
作者
Christen, Danilo
Schoenmann, Susan
Jermini, Mauro
Strasser, Reto J.
Defago, Genevieve
机构
[1] Swiss Fed Inst Technol, Inst Integrat Biol, CH-8092 Zurich, Switzerland
[2] Ctr Cadenazzo, Agroscope Changins Wadenswil Res Stn ACW, CH-6594 Contone, Switzerland
[3] Univ Geneva, Bioenerget Lab, CH-1254 Geneva, Switzerland
关键词
chlorophyll a fluorescence; drought stress; early detection; esca disease; eutypa dieback; JIP-test; Vitis vinifera; PHOTOSYSTEM-II; A FLUORESCENCE; ELECTRON-TRANSPORT; ENERGY-DISSIPATION; POLYPHASIC RISE; LIGHT STRESS; EUTYPA-LATA; PHOTOSYNTHESIS; PLANT; RESISTANCE;
D O I
10.1016/j.envexpbot.2007.02.003
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Esca disease, as well as other trunk diseases of grapevine, is an important wood disease that impedes the water transport in plants by clogging the xylem vessels. This type of effect is not detectable for years, due to the long latency time of the disease. In a field experiment, the susceptibility of Vitis vinifera cv. Cabernet sauvignon and Merlot to esca disease was evaluated by visual assessment of foliar symptoms and by necrosis and white rot indexes. C. sauvignon was highly susceptible and Merlot was tolerant to esca. The characteristics of fast chlorophyll a fluorescence transient were investigated in attached leaves by using the so-called JIP-test. The fluorescence transient was analyzed and plants without visible esca foliar symptoms were compared with those showing symptoms. In C. sauvignon, alteration of the photosynthetic apparatus could be detected 2 months before the appearance of foliar symptoms in autumn. To our knowledge, this is the first report of early detection of esca disease using a nondestructive method. For Merlot, only one JIP-test parameter was affected. However, when both cultivars were compared, the relationship of the performance index (defined by the density of reaction centers (RCs) and by the yields phi(Po) and psi(o); PIABS = [RC/ABS][phi(Po)/(1 - phi(Po))][psi(o)/(1 - psi(o))]) versus the calculated rate of the electron transfer [probability that an absorbed photon moves an electron further than Q(A)(-); phi(Eo) = phi(Po) psi(o) = (TRo/ABS)(ETo/TRo) = ETo/ABS = (1 - F-0/F-M)(l - V-J)] permitted us to separate the highest performing cultivar Merlot from the susceptible C. sauvignon. Also, the method used allowed us to detect modification of the photosystem II (PS II) performance in greenhouse-grown Riesling x Sylvaner after a drought stress. Finally, the comparison of the fluorescence transients of esca-affected and drought-stressed grapevines provided information on the differentiated functional-behavior patterns of PS II for the two stress types. These results suggest that esca infection cannot simply be interpreted as a water transport deficit through xylem dysfunction, but that other reaction mechanisms in the plants must be considered. The possibility to use fast chlorophyll a fluorescence monitoring as a wood decay early detection tool is discussed. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:504 / 514
页数:11
相关论文
共 61 条
[31]   Photosystem II efficiency and mechanisms of energy dissipation in iron-deficient, field-grown pear trees (Pyrus communis L.) [J].
Morales, F ;
Belkhodja, R ;
Abadía, A ;
Abadía, J .
PHOTOSYNTHESIS RESEARCH, 2000, 63 (01) :9-21
[32]   Esca (Black measles) and brown wood-streaking: Two old and elusive diseases of grapevines [J].
Mugnai, L ;
Graniti, A ;
Surico, G .
PLANT DISEASE, 1999, 83 (05) :404-418
[33]   FRUSTULOSINOL, AN ANTIBIOTIC METABOLITE OF STEREUM-FRUSTULOSUM - REVISED STRUCTURE OF FRUSTULOSIN [J].
NAIR, MSR ;
ANCHEL, M .
PHYTOCHEMISTRY, 1977, 16 (03) :390-392
[34]  
Papageorgiou G.C., 2004, Advances in Photosynthesis and Respiration, V19
[35]  
Pascoe I., 2000, Phytopathologia Mediterranea, V39, P68
[36]  
PEZET R, 1977, SWISS FEDERAL RES ST, P4
[37]   Action of UV and visible radiation on chlorophyll fluorescence from dark-adapted grape leaves (Vitis vinifera L.) [J].
Pfündel, EE .
PHOTOSYNTHESIS RESEARCH, 2003, 75 (01) :29-39
[38]  
POLIART C, 2000, ISOLEMENT CARACTERIS, P175
[39]  
Popovic R., 2003, Practical applications of chlorophyll fluorescence in plant biology, P151
[40]   BIOLOGICALLY-ACTIVE NATURAL ACETYLENIC-COMPOUNDS FROM EUTYPA-LATA (PERS-F) TUL [J].
RENAUD, JM ;
TSOUPRAS, G ;
TABACCHI, R .
HELVETICA CHIMICA ACTA, 1989, 72 (05) :929-932