Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity

被引:26
作者
Larsen, Jeppe Madura
Geisler, Carsten
Nielsen, Martin Weiss
Boding, Lasse
Von Essen, Marina
Hansen, Ann Kathrine
Skov, Lone
Bonefeld, Charlotte Menne
机构
[1] Univ Copenhagen, Fac Hlth Sci, Dept Int Hlth Immunol & Microbiol, Sect Expt Immunol, DK-2200 Copenhagen, Denmark
[2] Gentofte Univ Hosp, Dept Dermatol, DK-2820 Gentofte, Denmark
关键词
activation; contact hypersensitivity; flow cytometry; local lymph node assay; lymphocytes;
D O I
10.1111/j.1600-0536.2007.01230.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody-induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations. Objectives: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals. Patients/Methods: Mice were sensitized and/or challenged with 2,4-dinitrofluorobenzene or oxazolone. Using multi-parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes. Results: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system. Conclusions: Our study support recent findings that several cell types seem to be involved in CHS.
引用
收藏
页码:300 / 308
页数:9
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