Cloning and immunological characterization of the allergen Hel a 2 (profilin) from sunflower pollen

被引：45

作者：

Asturias, JA ^{[1
]}

Arilla, MC ^{[1
]}

Gómez-Bayón, N ^{[1
]}

Aguirre, M ^{[1
]}

Martínez, A ^{[1
]}

Palacios, R ^{[1
]}

Martínez, J ^{[1
]}

机构：

[1] IFIDESA, ARISTEGUI, Dept Res & Dev, Bilbao 48008, Spain

来源：

MOLECULAR IMMUNOLOGY | 1998年 / 35卷 / 08期

关键词：

profilin; pan-allergen; cloning; bacterial expression; recombinant allergen; affinity chromatography; IgE binding; monoclonal antibodies; Helianthus annuus;

D O I：

10.1016/S0161-5890(98)00036-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sunflower (Helianthus annuus) sensitization is not always related with occupational allergy. We have isolated the allergen profilin (Hel a 2) from this Compositae plant, cloned and sequenced five cDNAs encoding for full-length or partial Hel a 2. Natural sunflower profilin reacted with specific IgE in the 121 sera tested, at a frequency of 30.5%. Expression of the cDNA encoding Hel a 2 in Escherichia coli and a simple purification procedure by poly-L-proline chromatography allowed immunological characterization of the recombinant allergen. Binding of monoclonal antibodies against sunflower profilin revealed that some epitopes responsible for antigen-specific IgG production were not present in the recombinant allergen. High cross-reactivity has been found between recombinant Hel a 2 and profilins from other Compositae plants and also from botanically distant plants. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：469 / 478

页数：10

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