Flagellin as a biomarker for Bacillus subtilis strains;: Application to the DB9011 strain and the study of interspecific diversity in amino-acid sequences

被引：9

作者：

Asano, Y

Onishi, H

Tajima, K

Shinozawa, T ^{[1
]}

机构：

[1] Gunma Univ, Fac Engn, Dept Biol & Chem Engn, Kiryu, Gumma 3768515, Japan

[2] AHC Inc, Maebashi, Gumma 3710831, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 05期

关键词：

Bacillus subtilis DB9011; flagellin coding gene (hag); ITS-PCR;

D O I：

10.1271/bbb.65.1218

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polymerase chain reaction (PCR) for the flagellin central domain coding region (FCD-PCR) was applied to the detection and discrimination of Bacillus subtilis DB9011, a strain with useful functions in agriculture. Cross-reactions were observed in 4 B. subtilis strains with similar flagellin genes (hag). Alignment of partial amino-acid sequences of flagellin and the results of PCR for the 16S/23S rRNA spacer in 11 B. subtilis strains suggested the presence of a group including strains with antifungal activity (DB9011 and others).

引用

页码：1218 / 1222

页数：5

共 18 条

[1] Establishment of monoclonal antibodies specific for Bacillus subtilis DB9011 [J].