Phenazines and other redox-active antibiotics promote microbial mineral reduction

被引:292
作者
Hernandez, ME
Kappler, A
Newman, DK
机构
[1] CALTECH, Dept Geol & Planetary Sci, Pasadena, CA 91125 USA
[2] CALTECH, Dept Environm Sci & Engn, Pasadena, CA 91125 USA
关键词
D O I
10.1128/AEM.70.2.921-928.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Natural products with important therapeutic properties are known to be produced by a variety of soil bacteria, yet the ecological function of these compounds is not well understood. Here we show that phenazines and other redox-active antibiotics can promote microbial mineral reduction. Pseudomonas chlororaphis PCL1391, a root isolate that produces phenazine-1-carboxamide (PCN), is able to reductively dissolve poorly crystalline iron and manganese oxides, whereas a strain carrying a mutation in one of the phenazine-biosynthetic genes (phzB) is not; the addition of purified PCN restores this ability to the mutant strain. The small amount of PCN produced relative to the large amount of ferric iron reduced in cultures of P. chlororaphis implies that PCN is recycled multiple times; moreover, poorly crystalline iron (hydr)oxide can be reduced abiotically by reduced PCN. This ability suggests that PCN functions as an electron shuttle rather than an iron chelator, a finding that is consistent with the observation that dissolved ferric iron is undetectable in culture fluids. Multiple phenazines and the glycopeptidic antibiotic bleomycin can also stimulate mineral reduction by the dissimilatory iron-reducing bacterium Shewanella oneidensis MR1. Because diverse bacterial strains that cannot grow on iron can reduce phenazines, and because thermodynamic calculations suggest that phenazines have lower redox potentials than those of poorly crystalline iron (hydr)oxides in a range of relevant environmental pH (5 to 9), we suggest that natural products like phenazines may promote microbial mineral reduction in the environment.
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收藏
页码:921 / 928
页数:8
相关论文
共 59 条
[1]   Isolation and characterization of methanophenazine and function of phenazines in membrane-bound electron transport of Methanosarcina mazei Gol [J].
Abken, HJ ;
Tietze, M ;
Brodersen, J ;
Bäumer, S ;
Beifuss, U ;
Deppenmeier, U .
JOURNAL OF BACTERIOLOGY, 1998, 180 (08) :2027-2032
[2]   Microbial mobilization of arsenic from sediments of the Aberjona Watershed [J].
Ahmann, D ;
Krumholz, LR ;
Hemond, HF ;
Lovley, DR ;
Morel, FMM .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1997, 31 (10) :2923-2930
[3]  
[Anonymous], 1958, Bull Inst Chem Res
[4]   ANTIBIOTIC ACTION OF PYOCYANIN [J].
BARON, SS ;
ROWE, JJ .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1981, 20 (06) :814-820
[5]  
Beliaev AS, 1998, J BACTERIOL, V180, P6292
[6]   *KONSTITUTION VON DIHYDROPHENAZIN-DERIVATEN [J].
BIRKOFER, L .
CHEMISCHE BERICHTE-RECUEIL, 1952, 85 (11) :1023-1034
[7]   THE REDUCTION OF IRON OXIDE BY BACTERIA [J].
BROMFIELD, SM .
JOURNAL OF SOIL SCIENCE, 1954, 5 (01) :129-139
[8]   Geobacter metallireducens accesses insoluble Fe(III) oxide by chemotaxis [J].
Childers, SE ;
Ciufo, S ;
Lovley, DR .
NATURE, 2002, 416 (6882) :767-769
[9]   Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici [J].
Chin-A-Woeng, TFC ;
Bloemberg, GV ;
van der Bij, AJ ;
van der Drift, KMGF ;
Schripsema, J ;
Kroon, B ;
Scheffer, RJ ;
Keel, C ;
Bakker, PAHM ;
Tichy, HV ;
de Bruijn, FJ ;
Thomas-Oates, JE ;
Lugtenberg, BJJ .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (11) :1069-1077
[10]   Phenazines and their role in biocontrol by Pseudomonas bacteria [J].
Chin-A-Woeng, TFC ;
Bloemberg, GV ;
Lugtenberg, BJJ .
NEW PHYTOLOGIST, 2003, 157 (03) :503-523