共 46 条
Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis
被引:38
作者:

Kida, Yuichiro
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Hyogo, Grad Sch Life Sci, Kobe, Hyogo 6781297, Japan
Japan Sci & Technol Agcy, CREST, Kobe, Hyogo 6781297, Japan Univ Hyogo, Grad Sch Life Sci, Kobe, Hyogo 6781297, Japan

Morimoto, Fumiko
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Hyogo, Grad Sch Life Sci, Kobe, Hyogo 6781297, Japan Univ Hyogo, Grad Sch Life Sci, Kobe, Hyogo 6781297, Japan

Sakaguchi, Masao
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Hyogo, Grad Sch Life Sci, Kobe, Hyogo 6781297, Japan
Japan Sci & Technol Agcy, CREST, Kobe, Hyogo 6781297, Japan Univ Hyogo, Grad Sch Life Sci, Kobe, Hyogo 6781297, Japan
机构:
[1] Univ Hyogo, Grad Sch Life Sci, Kobe, Hyogo 6781297, Japan
[2] Japan Sci & Technol Agcy, CREST, Kobe, Hyogo 6781297, Japan
关键词:
D O I:
10.1083/jcb.200707050
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
During protein integration into the endoplasmic reticulum, the N-terminal domain preceding the type I signal-anchor sequence is translocated through a translocon. By fusing a streptavidin-binding peptide tag to the N terminus, we created integration intermediates of multispanning membrane proteins. In a cell-free system, N-terminal domain (N-domain) translocation was arrested by streptavidin and resumed by biotin. Even when N-domain translocation was arrested, the second hydrophobic segment mediated translocation of the downstream hydrophilic segment. In one of the defined intermediates, two hydrophilic segments and two hydrophobic segments formed a transmembrane disposition in a productive state. Both of the translocating hydrophilic segments were crosslinked with a translocon subunit, Sec61 alpha. We conclude that two translocating hydrophilic segment in a single membrane protein can span the membrane during multispanning topogenesis flanking the translocon. Furthermore, even after six successive hydrophobic segments entered the translocon, N-domain translocation could be induced to restart from an arrested state. These observations indicate the remarkably flexible nature of the translocon.
引用
收藏
页码:1441 / 1452
页数:12
相关论文
共 46 条
[21]
Reorientation of aquaporin-1 topology during maturation in the endoplasmic reticulum
[J].
Lu, Y
;
Turnbull, IR
;
Bragin, A
;
Carveth, K
;
Verkman, AS
;
Skach, WR
.
MOLECULAR BIOLOGY OF THE CELL,
2000, 11 (09)
:2973-2985

Lu, Y
论文数: 0 引用数: 0
h-index: 0
机构: Oregon Hlth Sci Univ, Div Mol Med, Portland, OR 97201 USA

Turnbull, IR
论文数: 0 引用数: 0
h-index: 0
机构: Oregon Hlth Sci Univ, Div Mol Med, Portland, OR 97201 USA

Bragin, A
论文数: 0 引用数: 0
h-index: 0
机构: Oregon Hlth Sci Univ, Div Mol Med, Portland, OR 97201 USA

Carveth, K
论文数: 0 引用数: 0
h-index: 0
机构: Oregon Hlth Sci Univ, Div Mol Med, Portland, OR 97201 USA

Verkman, AS
论文数: 0 引用数: 0
h-index: 0
机构: Oregon Hlth Sci Univ, Div Mol Med, Portland, OR 97201 USA

Skach, WR
论文数: 0 引用数: 0
h-index: 0
机构:
Oregon Hlth Sci Univ, Div Mol Med, Portland, OR 97201 USA Oregon Hlth Sci Univ, Div Mol Med, Portland, OR 97201 USA
[22]
SecYEG assembles into a tetramer to form the active protein translocation channel
[J].
Manting, EH
;
van der Does, C
;
Remigy, H
;
Engel, A
;
Driessen, AJM
.
EMBO JOURNAL,
2000, 19 (05)
:852-861

Manting, EH
论文数: 0 引用数: 0
h-index: 0
机构: Univ Groningen, Dept Microbiol, NL-9751 NN Haren, Netherlands

van der Does, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Groningen, Dept Microbiol, NL-9751 NN Haren, Netherlands

Remigy, H
论文数: 0 引用数: 0
h-index: 0
机构: Univ Groningen, Dept Microbiol, NL-9751 NN Haren, Netherlands

论文数: 引用数:
h-index:
机构:

Driessen, AJM
论文数: 0 引用数: 0
h-index: 0
机构: Univ Groningen, Dept Microbiol, NL-9751 NN Haren, Netherlands
[23]
Architecture of the ribosome-channel complex derived from native membranes
[J].
Ménétret, JF
;
Hegde, RS
;
Heinrich, SU
;
Chandramouli, P
;
Ludtke, SJ
;
Rapoport, TA
;
Akey, CW
.
JOURNAL OF MOLECULAR BIOLOGY,
2005, 348 (02)
:445-457

Ménétret, JF
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Hegde, RS
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Heinrich, SU
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Chandramouli, P
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Ludtke, SJ
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Rapoport, TA
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Akey, CW
论文数: 0 引用数: 0
h-index: 0
机构:
Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA
[24]
Structure of the E-coli protein-conducting channel bound to a translating ribosome
[J].
Mitra, K
;
Schaffitzel, C
;
Shaikh, T
;
Tama, F
;
Jenni, S
;
Brooks, CL
;
Ban, N
;
Frank, J
.
NATURE,
2005, 438 (7066)
:318-324

Mitra, K
论文数: 0 引用数: 0
h-index: 0
机构: New York State Dept Hlth, Wadsworth Ctr Labs & Res, Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA

Schaffitzel, C
论文数: 0 引用数: 0
h-index: 0
机构: New York State Dept Hlth, Wadsworth Ctr Labs & Res, Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA

Shaikh, T
论文数: 0 引用数: 0
h-index: 0
机构: New York State Dept Hlth, Wadsworth Ctr Labs & Res, Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA

Tama, F
论文数: 0 引用数: 0
h-index: 0
机构: New York State Dept Hlth, Wadsworth Ctr Labs & Res, Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA

Jenni, S
论文数: 0 引用数: 0
h-index: 0
机构: New York State Dept Hlth, Wadsworth Ctr Labs & Res, Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA

Brooks, CL
论文数: 0 引用数: 0
h-index: 0
机构: New York State Dept Hlth, Wadsworth Ctr Labs & Res, Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA

Ban, N
论文数: 0 引用数: 0
h-index: 0
机构: New York State Dept Hlth, Wadsworth Ctr Labs & Res, Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA

Frank, J
论文数: 0 引用数: 0
h-index: 0
机构: New York State Dept Hlth, Wadsworth Ctr Labs & Res, Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA
[25]
NHE6 protein possesses a signal peptide destined for endoplasmic reticulum membrane and localizes in secretory organelles of the cell
[J].
Miyazaki, E
;
Sakaguchi, M
;
Wakabayashi, S
;
Shigekawa, M
;
Mihara, K
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2001, 276 (52)
:49221-49227

Miyazaki, E
论文数: 0 引用数: 0
h-index: 0
机构: Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan

Sakaguchi, M
论文数: 0 引用数: 0
h-index: 0
机构: Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan

Wakabayashi, S
论文数: 0 引用数: 0
h-index: 0
机构: Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan

Shigekawa, M
论文数: 0 引用数: 0
h-index: 0
机构: Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan

Mihara, K
论文数: 0 引用数: 0
h-index: 0
机构: Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan
[26]
Structure of the mammalian ribosome-channel complex at 17 Å resolution
[J].
Morgan, DG
;
Ménétret, JF
;
Neuhof, A
;
Rapoport, TA
;
Akey, CW
.
JOURNAL OF MOLECULAR BIOLOGY,
2002, 324 (04)
:871-886

Morgan, DG
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Ménétret, JF
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Neuhof, A
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Rapoport, TA
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

Akey, CW
论文数: 0 引用数: 0
h-index: 0
机构: Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA
[27]
Fluorescence resonance energy transfer analysis of protein translocase -: SecYE from Thermus thermophilus HB8 forms a constitutive oligomer in membranes
[J].
Mori, H
;
Tsukazaki, T
;
Masui, R
;
Kuramitsu, S
;
Yokoyama, S
;
Johnson, AE
;
Kimura, Y
;
Akiyama, Y
;
Ito, K
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2003, 278 (16)
:14257-14264

Mori, H
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan

Tsukazaki, T
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan

Masui, R
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan

Kuramitsu, S
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan

Yokoyama, S
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan

Johnson, AE
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan

论文数: 引用数:
h-index:
机构:

Akiyama, Y
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan

Ito, K
论文数: 0 引用数: 0
h-index: 0
机构:
Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan
[28]
Protein translocation is mediated by oligomers of the SecY complex with one SecY copy forming the channel
[J].
Osborne, Andrew R.
;
Rapoport, Tom A.
.
CELL,
2007, 129 (01)
:97-110

Osborne, Andrew R.
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA

Rapoport, Tom A.
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA
[29]
Protein translocation by the Sec61/SecY channel
[J].
Osborne, AR
;
Rapoport, TA
;
van den Berg, B
.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY,
2005, 21
:529-550

Osborne, AR
论文数: 0 引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA

Rapoport, TA
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA

van den Berg, B
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA
[30]
Membrane integration of the second transmembrane segment of band 3 requires a closely apposed preceding signal-anchor sequence
[J].
Ota, K
;
Sakaguchi, M
;
Hamasaki, N
;
Mihara, K
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2000, 275 (38)
:29743-29748

Ota, K
论文数: 0 引用数: 0
h-index: 0
机构: Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan

Sakaguchi, M
论文数: 0 引用数: 0
h-index: 0
机构: Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan

Hamasaki, N
论文数: 0 引用数: 0
h-index: 0
机构: Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan

Mihara, K
论文数: 0 引用数: 0
h-index: 0
机构: Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan