Direct electrochemical oxidation of DNA on polycrystalline gold electrodes

被引:68
作者
Ferapontova, EE [1 ]
Dominguez, E
机构
[1] Novosibirsk IT Ctr, Grp Bioinformat, Novosibirsk 630011, Russia
[2] Lund Univ, Dept Analyt Chem, SE-22100 Lund, Sweden
[3] Univ Alcala de Henares, Fac Pharm, Dept Analyt Chem, E-28871 Alcala De Henares, Madrid, Spain
关键词
electrochemical oxidation of DNA; electrochemical impedance; guanine; gold;
D O I
10.1002/elan.200390079
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Electrochemical CV and SWV studies were performed with double stranded DNA from salmon testes (dsDNA) and single stranded DNAs, containing 25 nucleotides (ssDNA) directly adsorbed at polycrystalline An electrodes. A distinct oxidation peak at + 730 mV (SWV, scan rate 0.248 V s(-1)) or at + 730 - + 780 mV (CV, scan rate from 0.3 to 1 V s(-1)) was obtained with DNA-modified Au electrodes after a time-dependent prepolarization step at a positive potential value, i.e., at + 500 mV (vs. Ag \ AgCl), performed with the DNA-modified Au electrodes dipped in a blank buffer solution. No electrochemical activity was detected when ssDNA, containing no guanines, was used for adsorptive modification of the Au electrodes. Electrochemical impedance measurements registered a possible reorganization of the adsorbed DNA layer in the course of the prepolarization, accompanied by decreasing in-phase impedance. ne results enable us to relate the oxidation process observed at the DNA-modified Au electrodes with the oxidation of guanine residues in DNA.
引用
收藏
页码:629 / 634
页数:6
相关论文
共 43 条
[21]   DIFFERENTIAL-PULSE VOLTAMMETRIC DETERMINATION OF RNA AT THE PICOMOLE LEVEL IN THE PRESENCE OF DNA AND NUCLEIC-ACID [J].
PALECEK, E ;
FOJTA, M .
ANALYTICAL CHEMISTRY, 1994, 66 (09) :1566-1571
[22]   Past, present and future of nucleic acids electrochemistry [J].
Palecek, E .
TALANTA, 2002, 56 (05) :809-819
[23]   Detecting DNA hybridization and damage [J].
Palecek, E ;
Fojta, M .
ANALYTICAL CHEMISTRY, 2001, 73 (03) :74A-83A
[24]   ELECTROCHEMICAL OXIDATION OF DNA AT A GOLD MICROELECTRODE [J].
PANG, DW ;
QI, YP ;
WANG, ZL ;
CHENG, JK ;
WANG, JW .
ELECTROANALYSIS, 1995, 7 (08) :774-777
[25]   Modification of glassy carbon and gold electrodes with DNA [J].
Pang, DW ;
Zhang, M ;
Wang, ZL ;
Qi, YP ;
Cheng, JK ;
Liu, ZY .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1996, 403 (1-2) :183-188
[26]   Micromethod for the investigation of the interactions between DNA and redox active molecules [J].
Pang, DW ;
Abruña, HD .
ANALYTICAL CHEMISTRY, 1998, 70 (15) :3162-3169
[27]   How easily oxidizable is DNA? One-electron reduction potentials of adenosine and guanosine radicals in aqueous solution [J].
Steenken, S ;
Jovanovic, SV .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (03) :617-618
[28]   Reduction and oxidation of peptide nucleic acid and DNA at mercury and carbon electrodes [J].
Tomschik, M ;
Jelen, F ;
Havran, L ;
Trnková, L ;
Nielsen, PE ;
Palecek, E .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1999, 476 (01) :71-80
[29]   Electrochemical behavior of DNA at a silver electrode studied by cyclic and elimination voltammetry [J].
Trnková, L .
TALANTA, 2002, 56 (05) :887-894
[30]  
Walter P., 2017, MOL BIOL CELL