共 8 条
Cellular adhesion and neuronal excitability on functionalised diamond surfaces
被引:34
作者:
Ariano, P
Baldelli, P
Carbone, E
Gilardino, A
Lo Giudice, A
Lovisolo, D
Manfredotti, C
Novara, M
Sternschulte, H
Vittone, E
机构:
[1] Univ Turin, Expt Phys Dept, I-10125 Turin, Italy
[2] Univ Turin, NIS, I-10125 Turin, Italy
[3] Univ Turin, Dept Neurosci, I-10125 Turin, Italy
[4] Univ Augsburg, Inst Phys, D-8900 Augsburg, Germany
[5] Univ Turin, UdR, INFM, Nalt Inst Matter Phys, Turin, Italy
[6] Nanostructures Interfaces & Surfaces, Ctr Excellence, Dept Anim & Human Biol, Turin, Italy
关键词:
diamond film;
chemical vapour deposition;
biocompatibility;
biomaterials;
neurons;
D O I:
10.1016/j.diamond.2004.11.021
中图分类号:
T [工业技术];
学科分类号:
08 ;
摘要:
The resting or evoked activity of neuronal networks can be effectively monitored by using multielectrode arrays (MEA), which allow non-invasive extracellular stimulation and recording of electrical signals in parallel from multiple cells. Diamond possesses unique properties (biocompatibility, optical transparency, possibility of modifying the electronic and hydrophilic/hydrophobic properties at the nanoscale), which makes it a promising material to fabricate stable MEAs for long-term extracellular recordings of electrical and optical signals in living neurons. In order to explore the capability of diamond for fabricating MEAs as cell-based biosensors, we report here the first study on the adhesion and cell excitability (i.e., the ability of cells to generate and propagate trains of electrical impulses) on hydrogen (HTD)- and oxygen (OTD)-terminated diamond surfaces. Adhesion and functional properties of cultured rat hippocampal neurons and chick ciliary ganglia have been quantitatively evaluated using well-established biophysical techniques. Cells survive, adhere and maintain their electrical properties (synaptic activity, ion channels availability, Ca2+ signals during neuronal stimulation) for days provided that mixtures of adhesion molecules (poly-D-lysine, poly-DL-ornithine, laminin) are used to favour cell anchoring on diamond surface. (c) 2004 Elsevier B.V. All rights reserved.
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页码:669 / 674
页数:6
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