Pre-column derivatization of rofecoxib for determination in serum by HPLC

被引：8

作者：

Amini, M ^{[1
]}

Hamedani, MP ^{[1
]}

Vosooghi, M ^{[1
]}

Nabavi, M ^{[1
]}

Shafiee, A ^{[1
]}

机构：

[1] Univ Tehran Med Sci, Fac Pharm, Dept Med Chem & Pharmaceut Sci, Res Ctr, Tehran, Iran

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2005年 / 382卷 / 05期

关键词：

rofecoxib; HPLC; serum; UV detection;

D O I：

10.1007/s00216-005-3249-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An HPLC method for determination of rofecoxib in human serum is presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of the drug. Rofecoxib and the internal standard were extracted from serum using liquid-liquid extraction. Upon exposure to UV light, the drug was found to undergo a photocyclization reaction, giving a species with high absorbance. Validation of the method has been studied in the concentration range 10-500 ng ml(-1).

引用

页码：1265 / 1268

页数：4