Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly

被引：27

作者：

Calvo-Muñoz, ML

Dupont-Filliard, A

Billon, M

Guillerez, S

Bidan, G

Marquette, C

Blum, L

机构：

[1] CNRS, UJF, UMR 5819, CEA,Dept Rech Fondamentale Mat Condensee,Grp Elec, F-38054 Grenoble, France

[2] ASTEC, Apibio, F-38054 Grenoble, France

[3] Univ Lyon 1, CNRS, UMR 5013, UCB,Lab Genie Enzymat & Biomol, F-69622 Villeurbanne, France

来源：

BIOELECTROCHEMISTRY | 2005年 / 66卷 / 1-2期

关键词：

ABEI; ECL; polypyrrole; DNA; sensor; detection;

D O I：

10.1016/j.bioelechem.2004.04.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a single stranded DNA target (ODNt) has been used for the ECL detection of the hybridization between a complementary single stranded DNA probe (ODNp) covalently linked to a polypyrrole support and the ODNt. The ECL detection has been performed using a DNA sensor having a low surface concentration of ODNp probes, constituted of a polypyrrole copolymer electrosynthesized from a pyrrole-ODNp/ pyrrole monomer ratio of 1/20,000. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：139 / 143

页数：5

共 11 条

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