Fabricating large arrays of microwells with arbitrary dimensions and filling them using discontinuous dewetting

被引：274

作者：

Jackman, RJ ^{[1
]}

Duffy, DC ^{[1
]}

Ostuni, E ^{[1
]}

Willmore, ND ^{[1
]}

Whitesides, GM ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 11期

关键词：

D O I：

10.1021/ac971295a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes the fabrication of large (up to 45 cm(2)) arrays of microwells, with volumes as small as similar to 3 fL/well and densities as high as 10(7) wells/cm(2). These arrays of microwells are formed by casting an elastomer, poly(dimethylsiloxane) (PDMS), against "masters" prepared by photolithography; arrays of microwells in other polymers can be formed by using a master consisting of posts in PDMS. A straightforward technique, discontinuous dewetting, allows wells to be filled rapidly (typically on the order of 10(4) wells/s) and uniformly with a wide range of liquids. Several rudimentary strategies for addressing microwells are investigated, including electroosmotic pumping and gaseous diffusion.

引用

页码：2280 / 2287

页数：8