Regulation of pancreatic exocrine function in Otsuka Long-Evans Tokushima Fatty (OLETF) rats without gene expression of cholecystokinin-A receptor

被引：8

作者：

Funakoshi, A

Miyasaka, K

Shinozaki, H

Arita, Y

Nakano, I

Nawata, H

机构：

[1] Department of Gastroenterology, National Kyushu Cancer Center, Fukuoka

[2] Department of Clinical Physiology, Tokyo Metropol. Inst. of Gerontology, Tokyo

[3] Social Insurance Inatsuki Hospital, Fukuoka

[4] Third Dept. of Internal Medicine, Kyushu University, Fukuoka

来源：

INTERNAL MEDICINE | 1996年 / 35卷 / 04期

关键词：

cholecystokinin (CCK)-A receptor; polymerase chain reaction; pancreatic exocrine function; diabetes mellitus;

D O I：

10.2169/internalmedicine.35.249

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This work extends a recent observation that Otsuka Long-Evans Tokushima Fatty (OLETF) rats show a congenital defect of the cholecystokinin (CCK)-A receptor gene. Expression of CCK-A receptor mRNA in the pancreas, small intestine and brain were not detected in OLETF rats by the reverse transcriptase polymerase chain reaction. In vitro studies showed that the maximal effective concentrations of neuromedin C, acetylcholine and secretin for stimulation of amylase secretion were comparable in both strains, but that CCK-stimulated amylase secretion was observed only in Long-Evans Tokushima Otsuka (LETO) rats. Intracellular cytosolic Ca2+ movement stimulated by acetylcholine and neuromedin C was similar in both strains. In vivo studies showed that the pancreatic secretions in response to secretin and acetylcholine were not impaired in OLETF rats. However, protein responses to neuromedin C and 2-deoxy-D-glucose were impaired in OLETF rats. The findings suggest that pancreatic exocrine functions in OLETF rats are regulated by all neural and peptidergic agents except CCK.

引用

页码：249 / 256

页数：8

共 28 条

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