The dynamic machinery of mRNA elongation

被引：15

作者：

Armache, KJ ^{[1
]}

Kettenberger, H ^{[1
]}

Cramer, P ^{[1
]}

机构：

[1] Univ Munich, Dept Chem & Biochem, Gene Ctr, D-81377 Munich, Germany

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2005年 / 15卷 / 02期

关键词：

D O I：

10.1016/j.sbi.2005.03.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two complementary X-ray studies of the interaction between RNA polymerase 11 and nucleic acids have improved our understanding of mRNA elongation. These studies suggest how RNA polymerase 11 unwinds DNA, how it separates the RNA product from the DNA template and how it incorporates nucleoside triphosphate (NTP) substrates into the growing RNA chain. The tunable polymerase active center apparently allows repositioning of a catalytic metal ion, rotation of NTPs before their incorporation, RNA repositioning by a transcript cleavage factor, and modulation of enzyme activity by a bacterial small molecule regulator and its protein cofactor.

引用

页码：197 / 203

页数：7

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