Biochemical characterization of SFC-1, a class a carbapenem-hydrolyzing β-lactamase

被引：24

作者：

Fonseca, Fatima ^{[1
]}

Sarmento, Ana Cristina

Henriques, Isabel

Samyn, Bart

van Beeumen, Jozef

Domingues, Pedro

Domingues, Maria Rosario

Saavedra, Maria Jose

Correia, Antonio

机构：

[1] Univ Aveiro, Ctr Environm & Marine Studies, P-3810193 Aveiro, Portugal

[2] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal

[3] Univ Ghent, Dept Biochem Physiol & Microbiol, Lab Prot Biochem & Prot Engn, Ghent, Belgium

[4] Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal

[5] Univ Tras Os Montes & Alto Douro, Ctr Studies Anim & Vet Sci, Dept Vet Sci, P-5001801 Vila Real, Portugal

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2007年 / 51卷 / 12期

关键词：

D O I：

10.1128/AAC.00491-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The carbapenem-hydrolyzing P-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.

引用

页码：4512 / 4514

页数：3

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