Use of long-range repetitive element polymorphism-PCR to differentiate Bacillus anthracis strains

被引:28
作者
Brumlik, MJ [1 ]
Szymajda, U [1 ]
Zakowska, D [1 ]
Liang, XD [1 ]
Redkar, RJ [1 ]
Patra, G [1 ]
Del Vecchio, VG [1 ]
机构
[1] Univ Scranton, Inst Mol Biol & Med, Scranton, PA 18510 USA
关键词
D O I
10.1128/AEM.67.7.3021-3028.2001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The genome of Bacillus anthracis is extremely monomorphic, and thus individual strains have often proven to be recalcitrant to differentiation at the molecular level, Long-range repetitive element polymorphism-PCR (LR REP-PCR) was used to differentiate various B, anthracis strains. A single PCR primer derived from a repetitive DNA element was able to amplify variable segments of a bacterial genome as large as 10 kb, We were able to characterize five genetically distinct groups by examining 105 B, anthracis strains of diverse geographical origins. All B, anthracis strains produced fingerprints comprising seven to eight bands, referred to as "skeleton" bands, while one to three "diagnostic" bands differentiated between B, anthracis strains. LR REP-PCR fingerprints of B, anthracis strains showed very little in common with those of other closely related species such as B, cereus, B, thuringiensis, and B, mycoides, suggesting relative heterogeneity among the non-B, anthracis strains, Fingerprints from transitional non-B, anthracis strains, which possessed the B, anthracis chromosomal marker Ba813, scarcely resembled those observed for any of the five distinct B, anthracis groups that we have identified. The LR REP-PCR method described in this report provides a simple means of differentiating B, anthracis strains.
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页码:3021 / 3028
页数:8
相关论文
共 30 条
  • [11] The presence of intragenically located REP-like elements in Bacillus sporothermodurans is sufficient for REP-PCR typing
    Herman, L
    Heyndrickx, M
    [J]. RESEARCH IN MICROBIOLOGY, 2000, 151 (04) : 255 - 261
  • [12] REPETITIVE EXTRAGENIC PALINDROMIC SEQUENCES, MESSENGER-RNA STABILITY AND GENE-EXPRESSION - EVOLUTION BY GENE CONVERSION - A REVIEW
    HIGGINS, CF
    MCLAREN, RS
    NEWBURY, SF
    [J]. GENE, 1988, 72 (1-2) : 3 - 14
  • [13] Sensitive and rapid identification of biological threat agents
    Higgins, JA
    Ibrahim, MS
    Knauert, FK
    Ludwig, GV
    Kijek, TM
    Ezzell, JW
    Courtney, BC
    Henchal, EA
    [J]. FOOD AND AGRICULTURAL SECURITY: GUARDING AGAINST NATURAL THREATS AND TERRORIST ATTACKS AFFECTING HEALTH, NATIONAL FOOD SUPPLIES, AND AGRICULTURAL ECONOMICS, 1999, 894 : 130 - 148
  • [14] Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates
    Jackson, PJ
    Walthers, EA
    Kalif, AS
    Richmond, KL
    Adair, DM
    Hill, KK
    Kuske, CR
    Andersen, GL
    Wilson, KH
    HughJones, ME
    Keim, P
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (04) : 1400 - 1405
  • [15] Molecular evolution and diversity in Bacillus anthracis as detected by amplified fragment length polymorphism markers
    Keim, P
    Kalif, A
    Schupp, J
    Hill, K
    Travis, SE
    Richmond, K
    Adair, DM
    HughJones, M
    Kuske, CR
    Jackson, P
    [J]. JOURNAL OF BACTERIOLOGY, 1997, 179 (03) : 818 - 824
  • [16] Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis
    Keim, P
    Price, LB
    Klevytska, AM
    Smith, KL
    Schupp, JM
    Okinaka, R
    Jackson, PJ
    Hugh-Jones, ME
    [J]. JOURNAL OF BACTERIOLOGY, 2000, 182 (10) : 2928 - 2936
  • [17] Molecular epidemiology of an outbreak of febrile gastroenteritis caused by Listeria monocytogenes in cold-smoked rainbow trout
    Miettinen, MK
    Siitonen, A
    Heiskanen, P
    Haajanen, H
    Björkroth, KJ
    Korkeala, HJ
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (07) : 2358 - 2360
  • [18] Molecular characterization of Bacillus strains involved in outbreaks of anthrax in France in 1997
    Patra, G
    Vaissaire, J
    Weber-Levy, M
    Le Doujet, C
    Mock, M
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (11) : 3412 - 3414
  • [19] PATRA G, 1996, SALISBURY MED B SALI, P59
  • [20] Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA
    Ramisse, V
    Patra, G
    Garrigue, H
    Guesdon, JL
    Mock, M
    [J]. FEMS MICROBIOLOGY LETTERS, 1996, 145 (01) : 9 - 16