Photorespiratory metabolism of glyoxylate and formate in glycine-accumulating mutants of barley and Amaranthus edulis

被引:59
作者
Wingler, A [1 ]
Lea, PJ
Leegood, RC
机构
[1] Univ Sheffield, Robert Hill Inst, Sheffield S10 2TN, S Yorkshire, England
[2] Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England
[3] Univ Lancaster, Lancaster LA1 4YQ, England
基金
英国生物技术与生命科学研究理事会;
关键词
Amaranthus (mutant); C1-tetrahydrofolate synthase pathway; 10-formyltetrahydrofolate synthetase; glycine decarboxylase; Hordeum (mutant); photorespiration;
D O I
10.1007/s004250050512
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Glycine-accumulating mutants of barley (Hordeum vulgare L.) and Amaranthus edulis (Speg.), which lack the ability to decarboxylate glycine by glycine decarboxylase (GDC; EC 2.1.2.10, were used to study the significance of an alternative photorespiratory pathway of serine formation. In the normal photorespiratory pathway, 5,10-methylenetetrahydrofolate is formed in the reaction catalysed by GDC and transferred to serine by serine hydroxymethyltransferase. In an alternative pathway, glyoxylate could be decarboxylated to formate and formate could be converted into 5,10-methylenetetrahydrofolate in the Cl-tetrahydrofolate synthase pathway. In contrast to wild-type plants, the mutants showed a light-dependent accumulation of glyoxylate and formate, which was suppressed by elevated (0.7%) CO2 concentrations. After growth in air, the activity and amount of 10-formyltetrahydrofolate synthetase (FTHF synthetase; EC 6.3.4.4), the first enzyme of the conversion of formate into 5,10-methylenetetrahydrofolate, were increased in the mutants compared to the wild types. A similar increase in FTHF synthetase could be induced by incubating leaves of wild-type plants with glycine under illumination, but not in the dark. Experiments with C-14 showed that the barley mutants incorporated [C-14]formate and [2-C-14]glycollate into serine. Together, the accumulation of glyoxylate and formate under photorespiratory conditions, the increase in FTHF synthetase and the ability to utilise formate and glycollate for the formation of serine indicate that the mutants are able partially to compensate for the lack of GDC activity by bypassing the normal photorespiratory pathway.
引用
收藏
页码:518 / 526
页数:9
相关论文
共 43 条
[21]   THE ISOLATION AND CHARACTERIZATION OF A CATALASE-DEFICIENT MUTANT OF BARLEY (HORDEUM-VULGARE-L) [J].
KENDALL, AC ;
KEYS, AJ ;
TURNER, JC ;
LEA, PJ ;
MIFLIN, BJ .
PLANTA, 1983, 159 (06) :505-511
[22]   THE AFFINITY OF PEA COTYLEDON 10-FORMYLTETRAHYDROFOLATE SYNTHETASE FOR POLYGLUTAMATE SUBSTRATES [J].
KIRK, CD ;
IMESON, HC ;
ZHENG, LL ;
COSSINS, EA .
PHYTOCHEMISTRY, 1994, 35 (02) :291-296
[23]  
KIRKHAM P, 1995, SIGHT SOUND, V5, P10
[24]   A study of photorespiratory ammonia production in the C-4 plant Amaranthus edulis, using mutants with altered photosynthetic capacities [J].
Lacuesta, M ;
Dever, LV ;
MunozRueda, A ;
Lea, PJ .
PHYSIOLOGIA PLANTARUM, 1997, 99 (03) :447-455
[25]   THE REGULATION AND CONTROL OF PHOTORESPIRATION [J].
LEEGOOD, RC ;
LEA, PJ ;
ADCOCK, MD ;
HAUSLER, RE .
JOURNAL OF EXPERIMENTAL BOTANY, 1995, 46 :1397-1414
[26]   PHOTORESPIRATORY-N DONORS, AMINOTRANSFERASE SPECIFICITY AND PHOTOSYNTHESIS IN A MUTANT OF BARLEY DEFICIENT IN SERINE - GLYOXYLATE AMINOTRANSFERASE ACTIVITY [J].
MURRAY, AJS ;
BLACKWELL, RD ;
JOY, KW ;
LEA, PJ .
PLANTA, 1987, 172 (01) :106-113
[27]  
NAKAMURA Y, 1983, J BIOL CHEM, V258, P7631
[28]  
NOUR JM, 1991, J BIOL CHEM, V266, P18363
[29]  
NOUR JM, 1992, J BIOL CHEM, V267, P16292
[30]   FORMATE OXIDATION AND OXYGEN REDUCTION BY LEAF MITOCHONDRIA [J].
OLIVER, DJ .
PLANT PHYSIOLOGY, 1981, 68 (03) :703-705