Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IV

被引:246
作者
Boyle-Vavra, S
Ereshefsky, B
Wang, CC
Daum, RS
机构
[1] Univ Chicago, Infect Dis Sect, Dept Pediat, Chicago, IL 60637 USA
[2] TriServ Gen Hosp, Dept Pediat, Natl Def Med Ctr, Taipei, Taiwan
关键词
D O I
10.1128/JCM.43.9.4719-4730.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Methicillin-resistant Staphylococcus aureus (MRSA) isolates carry the methicillin resistance gene (mecA) on a horizontally transferred genetic element called the staphylococcal chromosome cassette mec (SCCmec). Community-acquired MRSA (CAMRSA) isolates usually carry SCCmec type IV. We previously reported that 76% of 17 CAMRSA isolates (multilocus sequence type 59) obtained from pediatric patients with skin and soft tissue infections (SSTI) from Taipei did not carry SCCmec types I to IV. We used DNA sequence analysis to determine that the element harbored by these nontypeable isolates is a novel subtype of SCCmec V called SCCmec V.,. It contains a ccrC recombinase gene variant (ccrC2) and mec complex C2. One SSTI isolate contained molecular features of SCCmec IV but also contained ccrC2 (a feature of SCCmec V-T), suggesting that it may harbor a composite SCCmec element. The genes lukS-PV and lukF-PV encoding the Panton-Valentine leukocidin (PVL) were present in all CAMRSA SSTI isolates whether they contained SCCmec type IV or VT. SCCmec V, was also present in 5 of 34 (14.7%) CAMRSA colonization isolates collected from healthy children from Taipei who lacked MRSA risk factors. Four (80%) of the these isolates contained lukS-PV and lukF-PV, as did I of 27 (3.7%) SCCmec IV-containing colonization isolates. A total of 63% (10 of 16) of the SSTI isolates and 61.7% (21 of 34) of the colonization isolates tested were resistant to at least four classes of non-p-lactam antimicrobials. SCCmec VT is a novel SCCmec variant that is found in multiply resistant CAMRSA strains with sequence type 59 in Taipei in association with the PVL leukotoxin genes.
引用
收藏
页码:4719 / 4730
页数:12
相关论文
共 42 条
  • [11] Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus
    Enright, MC
    Day, NPJ
    Davies, CE
    Peacock, SJ
    Spratt, BG
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) : 1008 - 1015
  • [12] Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients
    Gillet, Y
    Issartel, B
    Vanhems, P
    Fournet, JC
    Lina, G
    Bes, M
    Vandenesch, F
    Piémont, Y
    Brousse, N
    Floret, D
    Etienne, J
    [J]. LANCET, 2002, 359 (9308) : 753 - 759
  • [13] Community-acquired methicillin-resistant Staphylococcus aureus in children with no identified predisposing risk
    Herold, BC
    Immergluck, LC
    Maranan, MC
    Lauderdale, DS
    Gaskin, RE
    Boyle-Vavra, S
    Leitch, CD
    Daum, RS
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1998, 279 (08): : 593 - 598
  • [14] Community-acquired methicillin-resistant Staphylococcus aureus colonization in healthy children attending an outpatient pediatric clinic
    Hussain, FM
    Boyle-Vavra, S
    Daum, RS
    [J]. PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2001, 20 (08) : 763 - 767
  • [15] Novel type v staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC
    Ito, T
    Ma, XX
    Takeuchi, F
    Okuma, K
    Yuzawa, H
    Hiramatsu, K
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (07) : 2637 - 2651
  • [16] Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus
    Ito, T
    Katayama, Y
    Asada, K
    Mori, N
    Tsutsumimoto, K
    Tiensasitorn, C
    Hiramatsu, K
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (05) : 1323 - 1336
  • [17] Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains:: Role of IS431-mediated mecI deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus
    Katayama, Y
    Ito, T
    Hiramatsu, K
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (07) : 1955 - 1963
  • [18] Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia
    Lina, G
    Piémont, Y
    Godail-Gamot, F
    Bes, M
    Peter, MO
    Gauduchon, V
    Vandenesch, F
    Etienne, J
    [J]. CLINICAL INFECTIOUS DISEASES, 1999, 29 (05) : 1128 - 1132
  • [19] Risk factors and molecular analysis of community methicillin-resistant Staphylococcus aureus carriage
    Lu, PL
    Chin, LC
    Peng, CF
    Chiang, YH
    Chen, TP
    Ma, L
    Siu, LK
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (01) : 132 - 139
  • [20] Type 1 capsule genes of Staphylococcus aureus are carried in a staphylococcal cassette chromosome genetic element
    Luong, TT
    Ouyang, S
    Bush, K
    Lee, CY
    [J]. JOURNAL OF BACTERIOLOGY, 2002, 184 (13) : 3623 - 3629