Synthesis and characterization of a biospecific adsorbent containing bovine serum albumin as a ligand and its use for bilirubin retention

被引：21

作者：

Alvarez, C ^{[1
]}

Strumia, M ^{[1
]}

Bertorello, H ^{[1
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Quim Organ, RA-5000 Cordoba, Argentina

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2001年 / 49卷 / 1-3期

关键词：

specific sorbents; epoxy-activated gel; albumin-modified gel; bilirubin removal;

D O I：

10.1016/S0165-022X(01)00226-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Epoxy-activated gels from cross-linked polybutadiene-hydroxyethylmethacrylate (PB-HEMA) copolymer and epichlorohydrin (ECH) were prepared and characterized. Albumin was covalently bonded to the matrix and used as support of affinity chromatography in bilirubin (BR) retention experiments. PB-HEMA-ECH with different amounts of immobilized albumin (between 5.20 and 6.80 in mg/g dry gel) were obtained. Bilirubin retention of 3.10 mg/g of these beads was observed at 5 degreesC. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：649 / 656

页数：8

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