New differences between isoenzymes A and B from Candida rugosa lipase

被引：25

作者：

Hernaiz, MJ ^{[1
]}

SanchezMontero, JM ^{[1
]}

Sinisterra, JV ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC PHARM,DEPT ORGAN & PHARMACEUT CHEM,E-28040 MADRID,SPAIN

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 04期

关键词：

D O I：

10.1023/A:1018382313427

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Water adsorption isotherms of pure lipases A and B from Candida rugosa are different and can be used to distinguish between the isoenzymes. The maximum esterification yield (50%, 20 h) can be achieved at initial 0.9 < a(w) < 1.0. Lipase B is more stereoselective (49% yield, 98% enantiomeri excess) than lipase A (47% yield, 72% enantiomerci excess) but both isoenzymes mainly esterify the (S) 2(4-isobutylphenyl)propionic acid (Ibuprofen).

引用

页码：303 / 306

页数：4

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