The effect of stress upon hydrolysis adenine nucleotides in blood serum of rats

被引:8
作者
Böhmer, AE
Fürstenau, CR
Torres, ILS
Crema, L
Battastini, AMO
Dalmaz, C
Ferreira, MBC
Sarkis, JJF [1 ]
机构
[1] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, Anexo Lab, BR-90035003 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Farmacol, BR-90035003 Porto Alegre, RS, Brazil
关键词
apyrase 5 '-nucleotidase; stress; nucleotide hydrolysis; rat; blood serum;
D O I
10.1016/S0091-3057(03)00143-6
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
Alterations of enzyme activities involved in adenine nucleotide hydrolysis have been reported in spinal cord and blood serum after repeated restraint stress. On the other hand, no effect was observed in the spinal cord of rats after acute stress. In the present study, we investigated the effect of acute stress on the hydrolysis of adenine nucleotides in rat blood serum. Adult male Wistar rats were submitted to I h restraint stress and were sacrificed at 0, 6, 24 and 48 h. Increased ATP and ADP hydrolysis were observed in the blood serum of stressed rats 24 It after stress (58% and 54%, respectively, when compared to controls). On the other hand, the AMP hydrolysis was increased after 6 h (68% when compared to controls) and at 24 It (94% when compared to controls) after stress. The results suggest that altered activity of soluble enzymes in serum may be a biochemical marker for stress situations. (C) 2003 Published by Elsevier Inc.
引用
收藏
页码:467 / 471
页数:5
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