Combinatorial modification of degradable polymers enables transfection of human cells comparable to adenovirus

被引：138

作者：

Green, Jordan J.

Zugates, Gregory T.

Tedford, Nathan C.

Huang, Yu-Hung

Griffith, Linda G.

Lauffenburger, Douglas A.

Sawicki, Janet A.

Langer, Robert

Anderson, Daniel G.

机构：

[1] MIT, Canc Res Ctr, Cambridge, MA 02139 USA

[2] MIT, Div Biol Engn, Cambridge, MA 02139 USA

[3] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[4] Lankenau Inst Med Res, Wynnewood, PA 19096 USA

来源：

ADVANCED MATERIALS | 2007年 / 19卷 / 19期

关键词：

D O I：

10.1002/adma.200700371

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

End-modified poly(beta-amino ester)s, easy-to-synthesize degradable polymers, are able to deliver DNA to primary human cells at levels comparable to adenovirus and two orders of magnitude better than the commonly used non-viral vector, polyethylenimine. Small structural changes are found to affect multiple steps of gene delivery including the DNA binding affinity, nanoparticle size, intracellular DNA uptake, and final protein expression. In vivo, these polymer modifications enhance DNA delivery to ovarian tumors.

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页码：2836 / +

页数：8

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