Evidence for local immunosuppression and demonstration of c-myc amplification in pyothorax-associated lymphoma

被引：26

作者：

Yamato, H ^{[1
]}

Ohshima, K ^{[1
]}

Suzumiya, J ^{[1
]}

Kikuchi, M ^{[1
]}

机构：

[1] Fukuoka Univ, Sch Med, Dept Pathol, Jonan Ku, Fukuoka 8140180, Japan

来源：

HISTOPATHOLOGY | 2001年 / 39卷 / 02期

关键词：

PAL; c-myc; local immunosuppression; EBV; CGH; REL;

D O I：

10.1046/j.1365-2559.2001.01197.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Aims: Pyothorax-associated lymphoma (PAL) develops in the pleural cavity of patients with a long history of pyothorax. Epstein-Barr virus (EBV) is also involved in PAL, similar to lymphomas in immunodeficient patients. Here we examined T-lymphocyte subsets as well as c-myc and REL gene amplification in PAL tissues. Methods and results: We determined the number and distribution of CD4+ and CD8+ T-lymphocytes, to evaluate T-cells in the host immune reaction in seven cases of PAL. As controls, we also studied 10 cases of extranodal diffuse large B-cell lymphoma (DLBL) and 10 cases of nodal DLBL. Chromosomal imbalances in PAL were determined by using comparative genomic hybridization (CGH) analysis. The mean numbers of CD4+ and CD8+ and their ratio were significantly lower in PAI, than in nodal DLBL. CGH analysis of PAL showed amplification of the 8q24 chromosomal region. In addition, c-myc amplification was found in four cases of PAL by Southern blot analysis. Conclusions: Our results suggest that the development of PAL may involve a local immunosuppressive environment and that amplification of c-myc might promote tumour progression, as has been described in the development of Burkitt's lymphoma.

引用

页码：163 / 171

页数：9

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