Study of catalase immobilized on a silicate matrix for non-aqueous biocatalysis

被引：8

作者：

Horozova, E ^{[1
]}

Dimcheva, N ^{[1
]}

机构：

[1] Paisij Hilendarski Univ Plovdiv, Dept Phys Chem, BG-4000 Plovdiv, Bulgaria

来源：

CENTRAL EUROPEAN JOURNAL OF CHEMISTRY | 2005年 / 3卷 / 02期

关键词：

non-aqueous biocatalysis; immobilized catalase; silicate carrier; meta-Cloroperoxibenzoic acid;

D O I：

10.2478/BF02475996

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Catalytic activity of catalase (CAT) immobilized on a modified silicate matrix to mediate decomposition of meta-chloroperoxibenzoic acid (3-CPBA) in acetonitrile has been investigated by means of quantitative UV-spectrophotometry. Under the selected experimental conditions, the kinetic parameters: the apparent Michaelis constant (KM), the apparent maximum rate of enzymatic reaction (V-max(app)), the first order specific rate constants (k(sp)), the energy of activation (E-a) and the pre-exponential factor of the Arrhenius equation (Z(0)) were calculated. Conclusions regarding the rate-limiting step of the overall catalytic process were drawn from the calculated values of the Gibbs energy of activation Delta G*, the enthalpy of activation Delta H*, and the entropy of activation Delta S*. (C) Central European Science Journals. All rights reserved.

引用

页码：279 / 287

页数：9

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