A survey of zearalenone in corn using Romer Mycosep™ 224 column and high performance liquid chromatography

被引：39

作者：

Silva, CMG ^{[1
]}

Vargas, EA ^{[1
]}

机构：

[1] Minist Agr & Supply, Lab Mycotoxin Anal, BR-30380090 Belo Horizonte, MG, Brazil

来源：

FOOD ADDITIVES AND CONTAMINANTS | 2001年 / 18卷 / 01期

关键词：

zearalenone (ZEA); corn; Romer Mycosep (TM) 224 columns; high performance liquid chromatography;

D O I：

10.1080/02652030010002649

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A survey of zearalenone (ZEA) in corn from various regions of Brazil was carried out by the analysis of 380 corn samples, of which 30 samples (7.8%) were found to be contaminated in the range of 46.7-719 mug/kg. ZEA was extracted with acetonitrile-water (84:16, v/v), cleaned-up on a Romer Mycosep(TM) 224 column, separated, detected and quantified by high performance liquid chromatography (HPLC). The in-house method characteristics of linearity, accuracy, precision, and detection limit were defined by means of recovery tests with spiked corn samples in the range of 35.8-716 mug/kg and the analysis of a naturally-contaminated sample (n = 7). The mean recovery for ZEA was 99.4% and the relative standard deviation (RSD) varied from 0.7 to 26.6% in the range studied. The method has been shown to be accurate, quick and reliable for determination of zearalenone in corn.

引用

页码：39 / 45

页数：7

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