Preparative separation of rhein from Chinese traditional herb by repeated high-speed counter-current chromatography

被引：32

作者：

Wei, Y

Zhang, TY

Ito, Y

机构：

[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

[2] Beijing Inst New Technol Applicat, Beijing Res Ctr Separat & Purificat Technol Nat P, Beijing 100035, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1017卷 / 1-2期

关键词：

counter-current chromatography; rheum officinale; preparative chromatography; plant materials; pharmaceutical analysis; rhein;

D O I：

10.1016/j.chroma.2003.08.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography (HSCCC) was repeatedly used for isolation and purification of them from Rheum officinale Baill (Dahuang) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (3:7:5:5, v/v), which had been selected by analytical (HSCCC). Using two preparative units of the HSCCC centrifuge, about a 500 mg amount of the crude extract was separated, yielding 6.7 mg of them at a high purity of over 97%. (C) 2003 Elsevier B.V All rights reserved.

引用

页码：125 / 130

页数：6

共 8 条

[1] PREPARATION OF FUNCTIONALIZED JUGLONE ACETATES AND JUGLONES VIA 1,4-DIMETHOXYNAPHTHALENE DERIVATIVES - SYNTHESIS OF ANTHRAQUINONES RELATED TO RHEIN AND ALOE EMODIN [J].