Application of analytical and preparative high-speed counter-current chromatography for separation of lycopene from crude extract of tomato paste

被引：68

作者：

Wei, Y

Zhang, TY

Xu, GQ

Ito, Y

机构：

[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

[2] Beijing Inst New Technol, Beijing Res Ctr Separat & Purificat Technol Nat P, Beijing 100035, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 929卷 / 1-2期

关键词：

tomato paste; counter-current chromatography; lycopene;

D O I：

10.1016/S0021-9673(01)01177-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lycopene was isolated from 100 mg of crude extract of tomato paste containing about 9% of lycopene. Analytical high-speed counter-current chromatography (HSCCC) was first used for the systematic selection of the two-phase solvent system. Then preparative HSCCC separation was performed with a nonaqueous solvent system composed of n-hexane-dichloromethane-acetonitrile at an optimum volume ratio of 10:3.5:6.5. This yielded 8.6 mg of lycopene at over 98.5% purity as determined by HPLC analysis. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：169 / 173

页数：5

共 20 条

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