Differential roles for the E2A activation domains in B lymphocytes and macrophages

被引：21

作者：

Bhalla, Savita ^{[1
]}

Spaulding, Christina ^{[1
]}

Brumbaugh, Rachel L. ^{[1
]}

Zagort, Derek E. ^{[1
]}

Massari, Mark E. ^{[3
]}

Murre, Cornelis ^{[3
]}

Kee, Barbara L. ^{[1
,2
]}

机构：

[1] Univ Chicago, Dept Pathol, Chicago, IL 60637 USA

[2] Univ Chicago, Comm Immunol Canc Biol & Dev Biol, Chicago, IL 60637 USA

[3] Univ Calif San Diego, Dept Biol, La Jolla, CA 92190 USA

来源：

JOURNAL OF IMMUNOLOGY | 2008年 / 180卷 / 03期

关键词：

D O I：

10.4049/jimmunol.180.3.1694

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The E2A gene encodes two E protein/class I basic helix-loop-helix transcription factors, E12 and E47, that are essential for B lymphopoiesis. In addition to the DNA-binding and protein dimerization domain, the E proteins share two highly conserved transcription activation domains. In this study, we show that both activation domains are required for optimal E2A-dependent transcription. Surprisingly, however, neither activation domain is required for E2A to rescue B lymphopoiesis from E2A(-/-) hemopoietic progenitors, although the N terminus of E2A, which harbors some transcription capacity, is required. Therefore, the E protein activation domains function redundantly in promoting B cell development. In contrast, the N-terminal activation domain, AD1, is required for a newly described ability of E2A to suppress macrophage development in vitro. Our findings demonstrate distinct functionalities for the E protein activation domains in B lymphocytes and macrophages.

引用

页码：1694 / 1703

页数：10

共 44 条

[21] E proteins and Notch signaling cooperate to promote T cell lineage specification and commitment [J].