The distribution of the tail moments in single cell gel electrophoresis (comet assay) obeys a chi-square (χ2) not a gaussian distribution

被引：97

作者：

Bauer, E ^{[1
]}

Recknagel, RD ^{[1
]}

Fiedler, U ^{[1
]}

Wollweber, L ^{[1
]}

Bock, C ^{[1
]}

Greulich, KO ^{[1
]}

机构：

[1] Inst Mol Biotechnol, D-07708 Jena, Germany

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1998年 / 398卷 / 1-2期

关键词：

single cell gel electrophoresis; comet assay; mathematical statistics; chi-square distribution;

D O I：

10.1016/S0027-5107(97)00246-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The parameter tail moment in single cell gel electrophoresis (comet assay) is calculated as the product of the two values: the percentage of DNA in the comet tail and the tail length in mu m. Experiments were performed with cultured mammalian cells: B-Lymphoblasts, epithelial cells of a kidney tissue and a plate-epithelial cell line of a human carcinoma. They were irradiated in suspension with UV A at lambda = 343 nm, generated by an excimer laser-pumped dye laser. DNA migration was assessed and analysed. It is demonstrated that the distribution of the tail moments can be fitted by a chi(2) (chi-square) distribution, whereas the factors of the product tail moment tend to be normally distributed. From this result, consequences for the statistical evaluation of the results can arise, especially for the computation of the confidence limits and for the valuation of the parameter tail moment from other comet assay experiments. (C) 1998 Elsevier Science B.V.

引用

页码：101 / 110

页数：10

共 30 条

[1] Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays [J].