UV-B-LASER-INDUCED DNA-DAMAGE IN LYMPHOCYTES OBSERVED BY SINGLE-CELL GEL-ELECTROPHORESIS

被引：22

作者：

DEWITH, A ^{[1
]}

LEITZ, G ^{[1
]}

GREULICH, KO ^{[1
]}

机构：

[1] INST MOLEC BIOTECHNOL,D-100813 JENA,GERMANY

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1994年 / 24卷 / 01期

关键词：

LYMPHOCYTES; XECL LASER IRRADIATION; COMET ASSAY; SINGLE-CELL GEL ELECTROPHORESIS; DNA STRAND BREAKS;

D O I：

10.1016/1011-1344(94)07007-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human lymphocytes from a cell culture were irradiated with 12 mJ pulses (524 J m(-2)) of an XeCl excimer laser (308 nm). DNA strand breaks were analysed with the ''comet assay'', a single-cell method. The DNA damage of individual cells could be investigated depending on the energy and the irradiated volume. DNA damage could be detected already after a single laser pulse of 12 mJ. Since the irradiation time is short compared with the DNA repair time, DNA damage is solely dependent on the total number of photons. A quantitative evaluation of the number of damaged cells vs. number of photons can be best explained by a detection threshold model. The calculated threshold is 1.25 x 10(8) photons cell(-1). From this and the principal sensitivity of the comet assay, one can estimate that the upper limit for DNA damaging efficiency is 400 000 photons.

引用

页码：47 / 53

页数：7

共 21 条

[1] ASSESSMENT OF RADIATION-INDUCED DNA STRAND BREAKS AND THEIR REPAIR IN UNLABELED CELLS [J].