Biological effects of a sulfated-polysaceharide isolated from the marine red algae Champia feldmannii

被引:47
作者
Sampaio Assreuy, Ana Maria [1 ]
Gomes, Daniel Magalhaes [1 ]
Josino da Silva, Michelle Soares [1 ]
Torres, Valeska Martins [2 ]
Lima Siquera, Rommulo Celly [1 ]
Pires, Alana de Freitas [1 ]
Criddle, David Neil [3 ]
Nunes de Alencar, Nylane Maria [4 ]
Cavada, Benildo Sousa [5 ]
Sampaio, Alexandre Holanda [2 ]
Lobo Farias, Wladimir Ronald [2 ]
机构
[1] Univ Estadual Ceara, Inst Super Ciencias Biomed, BR-60740000 Fortaleza, Ceara, Brazil
[2] Univ Fed Ceara, Dept Engn Pesca, BR-60356000 Fortaleza, Ceara, Brazil
[3] Univ Liverpool, Physiol Lab, MRC Secretory Control Res Grp, Liverpool L69 3BX, Merseyside, England
[4] Univ Fed Ceara, Dept Fisiol & Farmacol, BR-60451970 Fortaleza, Ceara, Brazil
[5] Univ Fed Ceara, Dept Bioquim & Biol Mol, BR-60455970 Fortaleza, Ceara, Brazil
关键词
sulfated-polysaccharide; Champia feldmannii; inflammation; nociception; coagulation; marine algae;
D O I
10.1248/bpb.31.691
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Sulfated-polysaccharides are exploited as antithrombotic and anticoagulant agents and suggested to be immunostimulants. The sulfated-polysaccharide isolated from the red-marine-algae Champia feldmannii (Cf-PLS) was purified by ion exchange chromatography and tested in experimental protocols of coagulation, inflammation (in Wistar rats) and nociception (in Swiss mice). Cf-PLS was tested Lv. for its anti-inflammatory activity in the paw-edema induced by classical inflammatory stimuli and s.c. for its pro-inflammatory activity in the paw-edema and peritonitis models. The anticoagulant activity was evaluated by the test of partial thromboplastin activation time (aPTT) and the antinociceptive effect in the writhing-test. Cf-PLS was not anti-inflammatory, but rather induced maximal edematogenic activity at 0.9mg/kg (1.01 +/- 0.030X0.06 +/- 0.03ml) compared to controls (0.06 +/- 0.03 ml), increased vascular-permeability (38.44 +/- 12.63 X 11.29 +/- 3.91 mu/g) and stimulated nentrophil migration (3.348 +/- 295X307 +/- 99cells/mu l) 1h after injection. Cf-PLS was also antinociceptive (6.6 +/- 1.28X33 +/- 1.44 writhes) and extended human plasma coagulation time by 3 times. Our data suggest that this molecule may be an important immunostimulant.
引用
收藏
页码:691 / 695
页数:5
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