Biological effects of a sulfated-polysaceharide isolated from the marine red algae Champia feldmannii

被引：47

作者：

Sampaio Assreuy, Ana Maria ^{[1
]}

Gomes, Daniel Magalhaes ^{[1
]}

Josino da Silva, Michelle Soares ^{[1
]}

Torres, Valeska Martins ^{[2
]}

Lima Siquera, Rommulo Celly ^{[1
]}

Pires, Alana de Freitas ^{[1
]}

Criddle, David Neil ^{[3
]}

Nunes de Alencar, Nylane Maria ^{[4
]}

Cavada, Benildo Sousa ^{[5
]}

Sampaio, Alexandre Holanda ^{[2
]}

Lobo Farias, Wladimir Ronald ^{[2
]}

机构：

[1] Univ Estadual Ceara, Inst Super Ciencias Biomed, BR-60740000 Fortaleza, Ceara, Brazil

[2] Univ Fed Ceara, Dept Engn Pesca, BR-60356000 Fortaleza, Ceara, Brazil

[3] Univ Liverpool, Physiol Lab, MRC Secretory Control Res Grp, Liverpool L69 3BX, Merseyside, England

[4] Univ Fed Ceara, Dept Fisiol & Farmacol, BR-60451970 Fortaleza, Ceara, Brazil

[5] Univ Fed Ceara, Dept Bioquim & Biol Mol, BR-60455970 Fortaleza, Ceara, Brazil

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2008年 / 31卷 / 04期

关键词：

sulfated-polysaccharide; Champia feldmannii; inflammation; nociception; coagulation; marine algae;

D O I：

10.1248/bpb.31.691

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Sulfated-polysaccharides are exploited as antithrombotic and anticoagulant agents and suggested to be immunostimulants. The sulfated-polysaccharide isolated from the red-marine-algae Champia feldmannii (Cf-PLS) was purified by ion exchange chromatography and tested in experimental protocols of coagulation, inflammation (in Wistar rats) and nociception (in Swiss mice). Cf-PLS was tested Lv. for its anti-inflammatory activity in the paw-edema induced by classical inflammatory stimuli and s.c. for its pro-inflammatory activity in the paw-edema and peritonitis models. The anticoagulant activity was evaluated by the test of partial thromboplastin activation time (aPTT) and the antinociceptive effect in the writhing-test. Cf-PLS was not anti-inflammatory, but rather induced maximal edematogenic activity at 0.9mg/kg (1.01 +/- 0.030X0.06 +/- 0.03ml) compared to controls (0.06 +/- 0.03 ml), increased vascular-permeability (38.44 +/- 12.63 X 11.29 +/- 3.91 mu/g) and stimulated nentrophil migration (3.348 +/- 295X307 +/- 99cells/mu l) 1h after injection. Cf-PLS was also antinociceptive (6.6 +/- 1.28X33 +/- 1.44 writhes) and extended human plasma coagulation time by 3 times. Our data suggest that this molecule may be an important immunostimulant.

引用

页码：691 / 695

页数：5

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