Immunoenzymatic detection of the new proliferation associated protein p100 by means of a cellular ELISA: specific detection of cells in cell cycle phases S, G2 and M

In vitro proliferation assays are widely used in biomedical research. We describe the immunoenzymatic (ELISA) detection of a recently described proliferation associated protein (p100) by means of a new monoclonal mouse IgG1 antibody (Ki-S2). P100 is a 100 kDa nuclear protein that is specifically detected during the cell cycle phases S, G2 and M. Comparative studies on lectin-stimulated leukocytes using H-3-thymidine labelling and Ki-67 antibodies revealed a statistically significant positive correlation. Since p100 is absent in GO and G1 cells, its detection permits the precise and specific measurement of actual cell cycle events under culture conditions. (C) 1999 Elsevier Science B.V. AU rights reserved.