Immunoenzymatic detection of the new proliferation associated protein p100 by means of a cellular ELISA: specific detection of cells in cell cycle phases S, G2 and M

被引：12

作者：

Frahm, SO

Rudolph, P

Dworeck, C

Zott, B

Heidebrecht, H

Steinmann, J

Neppert, J

Parwaresch, R

机构：

[1] Univ Kiel, Dept Gen Pathol & Hematopathol, D-24105 Kiel, Germany

[2] Dept Transfus Med, D-24105 Kiel, Germany

[3] Univ Kiel, Dept Immunol, D-24105 Kiel, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1999年 / 223卷 / 02期

关键词：

proliferation; ELISA; Ki-S2; p100; Ki-67;

D O I：

10.1016/S0022-1759(98)00217-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro proliferation assays are widely used in biomedical research. We describe the immunoenzymatic (ELISA) detection of a recently described proliferation associated protein (p100) by means of a new monoclonal mouse IgG1 antibody (Ki-S2). P100 is a 100 kDa nuclear protein that is specifically detected during the cell cycle phases S, G2 and M. Comparative studies on lectin-stimulated leukocytes using H-3-thymidine labelling and Ki-67 antibodies revealed a statistically significant positive correlation. Since p100 is absent in GO and G1 cells, its detection permits the precise and specific measurement of actual cell cycle events under culture conditions. (C) 1999 Elsevier Science B.V. AU rights reserved.

引用

页码：147 / 153

页数：7