Cadmium-induced oxidative stress and DNA damage in kidney of pregnant female rats

被引：43

作者：

Chater, Sihem ^{[1
,2
,3
]}

Douki, Thierry ^{[3
]}

Garrel, Catherine ^{[2
]}

Favier, Alain ^{[2
,3
]}

Sakly, Mohsen ^{[1
]}

Abdelmelek, Hafedh ^{[1
]}

机构：

[1] Fac Sci Bizerte, Lab Physiol Integree, Zarzouna 7021, Tunisia

[2] Hop La Tronche, Dept Biol Integree, Lab Stress Oxydant, F-38700 Grenoble, France

[3] CEA Grenoble, Lab Les Acides Nucl, DRFMC SCIB, F-38054 Grenoble 9, France

来源：

COMPTES RENDUS BIOLOGIES | 2008年 / 331卷 / 06期

关键词：

cadmium; oxidative stress; 8-oxodGuo; rat;

D O I：

10.1016/j.crvi.2008.03.009

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In the present study, we have investigated the influence of sub-acute treatment with cadmium (Cd) on some parameters indicative of oxidative stress and DNA damage in tissues of pregnant female rats. Pregnant female rats (n = 6) were injected subcutaneously, daily with a dose of cadmium chloride of 3 mg/kg body weight (b.w.) from day 6 to day 19 of pregnancy, and they were allowed to deliver normally. MDA level and GPx, CAT and SOD activities were used as markers of oxidative stress in liver and kidney. The 8-oxo-dG level was measured by the HPLC-EC system. Cd treatment increased MDA (+116%, p < 0.01) in kidney. Moreover, Cd treatment also decreased CuZn-SOD (-11%, p < 0.05) and GSH level (-52%, p < 0.05) in kidney. Treated rats displayed an increase of the liver metallothionein (MT) level. Induction of MT in liver was probably implicated in the detoxification of Cd. The high level of Cd (3 mg/kg) used in the present study is partially neutralized by MT in liver, whereas the free fraction could be implicated in the oxidative stress and DNA oxidation observed in kidney. Cd treatment failed to alter 8-oxodGuo, indicating the absence of DNA oxidation in liver; by contrast, the same treatment increased the 8-oxodGuo level (+51%, p < 0.05) in the kidney of pregnant female rats, indicating an oxidative stress associated with DNA damage only in kidney.

引用

页码：426 / 432

页数：7

共 49 条

[1] INHIBITION OF HYDROXYL-RADICAL-GENERATED DNA-DEGRADATION BY METALLOTHIONEIN [J].

ABEL, J ;

DERUITER, N .

TOXICOLOGY LETTERS, 1989, 47 (02) :191-196

[2]

Aebi H., 1983, Methods in Enzymatic Analysis, P673, DOI [10.1016/B978-0-12-091302-2.50032-3, DOI 10.1016/B978-0-12-091302-2.50032-3]

[3]

Akerboom T P, 1981, Methods Enzymol, V77, P373

[4] ENHANCEMENT OF RAT AND HUMAN PHAGOCYTE SUPEROXIDE ANION RADICAL PRODUCTION BY CADMIUM INVITRO [J].

AMORUSO, MA ;

WITZ, G ;

GOLDSTEIN, BD .

TOXICOLOGY LETTERS, 1982, 10 (2-3) :133-138

[5] Induction of oxidative stress by chronic administration of sodium dichromate [chromium VI] and cadmium chloride [cadmium II] to rats [J].

Bagchi, D ;

Vuchetich, PJ ;

Bagchi, M ;

Hassoun, EA ;

Tran, MX ;

Tang, L ;

Stohs, SJ .

FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (03) :471-478

[6] STRUCTURAL-PROPERTIES OF THE ZINC SITE IN CU,ZN-SUPEROXIDE DISMUTASE - PERTURBED ANGULAR-CORRELATION OF GAMMA-RAY SPECTROSCOPY ON THE CU, CD-111-SUPEROXIDE DISMUTASE DERIVATIVE [J].

BAUER, R ;

DEMETER, I ;

HASEMANN, V ;

JOHANSEN, JT .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 94 (04) :1296-1302

[7] INTERACTIONS IN METAL CARCINOGENICITY [J].

BEYERSMANN, D .

TOXICOLOGY LETTERS, 1994, 72 (1-3) :333-338

[8] Effects of dietary vitamin E and selenium on antioxidative defense mechanisms in the liver of rats treated with high doses of glucocorticoid [J].

Beytut, E ;

Aksakal, M .

BIOLOGICAL TRACE ELEMENT RESEARCH, 2003, 91 (03) :231-241

[9]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[10] Effect of short-term ethanol administration on cadmium retention and bioelement metabolism in rats continuously exposed to cadmium [J].

Bróska, MM ;

Moniuszko-Jakoniuk, J ;

Jurczuk, M ;

Galazyn-Sidorczuk, M ;

Rogalska, J .

ALCOHOL AND ALCOHOLISM, 2000, 35 (05) :439-445

← 1 2 3 4 5 →