Preparation and evaluation of 125I-aflatoxin B1

被引：4

作者：

Korde, A ^{[1
]}

Banerjee, S ^{[1
]}

Pillai, MRA ^{[1
]}

Venkatesh, M ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr, Radiopharmaceut Div, Bombay 400085, Maharashtra, India

来源：

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY | 2001年 / 250卷 / 02期

关键词：

Aflatoxins - Nuclear magnetic resonance spectroscopy;

D O I：

10.1023/A:1017998202131

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Aflatoxin B-1 (AfB(1)), present in fungus infested crops is highly carcinogenic and is measured by immunoassays. I-125 labeled aflatoxin B-1 is a key reagent for development of radioimmunoassay (RIA) which exhibits less interference and better sensitivity than other immunoassays. Since AfB(1) lacks suitable functional groups for radiolabeling, an oxime derivative of AfB(1) was synthesised and evaluated by UV-spectrophotometry and H-1 NMR spectroscopy. I-125-histamine was conjugated to AfB(1) oxime by mixed anhydride method and purified by solvent extraction followed by TLC. The tracer obtained was immunoreactive, stable as ethanolic solution and could be used in RIA.

引用

页码：231 / 237

页数：7

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