Synthesis and characterization of extended length alkyl stationary phases for liquid chromatography with application to the separation of carotenoid isomers

被引：25

作者：

Bell, CM ^{[1
]}

Sander, LC ^{[1
]}

Fetzer, JC ^{[1
]}

Wise, SA ^{[1
]}

机构：

[1] CHEVRON RES & TECHNOL CO,RICHMOND,CA 94802

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 753卷 / 01期

关键词：

stationary phases; LC; carotenoids; carotenes;

D O I：

10.1016/S0021-9673(96)00562-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, long-chain (similar to C-34) alkyl-bonded stationary phase for liquid chromatography (LC) was synthesized and utilized for the separation of carotenoid mixtures. This stationary phase exhibited increased selectivity for certain cis isomers of alpha- and beta-carotene as compared to the recently reported C-30 stationary phase. The new stationary phase was synthesized using two bonding strategies: traditional polymeric synthesis and a surface-polymerization synthesis, with the latter yielding columns with improved resolution for carotenoids. A natural extract with a high degree of cis isomerization was well separated with this long-chain stationary phase.

引用

页码：37 / 45

页数：9

共 61 条

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