Mitochondrial glycosidic residues contribute to the interaction between ruthenium amine complexes and the calcium uniporter

被引：4

作者：

Correa, F ^{[1
]}

Zazueta, C ^{[1
]}

机构：

[1] Inst Nacl Cardiol Ignacio Chavez, Dept Bioquim, Mexico City 14080, DF, Mexico

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2005年 / 272卷 / 1-2期

关键词：

calcium uniporter; glycoproteins; mitochondria; ruthenium complex inhibitors;

D O I：

10.1007/s11010-005-6754-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The role of glycosidic residues in the inhibitory properties of ruthenium complexes on mitochondrial calcium uptake was determined in mitoplasts. Our results showed that the binding and inhibitory properties of ruthenium amine complexes were modified when mitoplasts were exposed to N-glycosidase F action, but calcium uptake was not altered. N-linked proteins of the mitochondrial inner membrane were identified. We detected an 18-kDa protein that binds labeled Ru-360 under control conditions, but failed to bind the inhibitor after deglycosilation. A relationship between this protein and the action of ruthenium amine inhibitors of the mitochondrial uniporter is proposed.

引用

页码：55 / 62

页数：8

共 34 条

[11] Calcium and mitochondria [J].

Gunter, TE ;

Yule, DI ;

Gunter, KK ;

Eliseev, RA ;

Salter, JD .

FEBS LETTERS, 2004, 567 (01) :96-102

[12] COMPARATIVE DISTRIBUTION OF CYTOCHROME-OXIDASE, SUCCINATE PERMEASE, AND FIXED ANIONIC SITES ON INTACT INNER MITOCHONDRIAL-MEMBRANE - POLYCATIONIC FERRITIN AS A VISUALLY DETECTABLE METABOLIC INHIBITOR [J].

HACKENBROCK, CR .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1975, 170 (01) :139-148

[13] Dynamic O-linked glycosylation of nuclear and cytoskeletal proteins [J].

Hart, GW .

ANNUAL REVIEW OF BIOCHEMISTRY, 1997, 66 :315-335

[14]

IGVABVOA I, 1991, J BIOL CHEM, V266, P4283

[15] ISOLATION AND CHARACTERIZATION OF GLYCOPEPTIDES FROM RAT-LIVER MITOCHONDRIA [J].

ITOH, N ;

KAWASAKI, T ;

YAMASHIN.I .

JOURNAL OF BIOCHEMISTRY, 1974, 76 (03) :459-466

[16] EVIDENCE FOR A CONFORMATIONAL CHANGE IN DEGLYCOSYLATED GLYCOPROTEIN HORMONES [J].

KEUTMANN, HT ;

JOHNSON, L ;

RYAN, RJ .

FEBS LETTERS, 1985, 185 (02) :333-338

[17] The mitochondrial calcium uniporter is a highly selective ion channel [J].

Kirichok, Y ;

Krapivinsky, G ;

Clapham, DE .

NATURE, 2004, 427 (6972) :360-364

[18] STRUCTURES AND FUNCTIONS OF THE SUGAR CHAINS OF GLYCOPROTEINS [J].

KOBATA, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 209 (02) :483-501

[19] Oxygen-bridged dinuclear ruthenium amine complex specifically inhibits Ca2+ uptake into mitochondria in vitro and in situ in single cardiac myocytes [J].

Matlib, MA ;

Zhou, Z ;

Knight, S ;

Ahmed, S ;

Choi, KM ;

Krause-Bauer, J ;

Phillips, R ;

Altschuld, R ;

Katsube, Y ;

Sperelakis, N ;

Bers, DM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (17) :10223-10231

[20] ISOLATION AND PROPERTIES OF CA-2+-TRANSPORTING GLYCOPROTEIN AND PEPTIDE FROM BEEF-HEART MITOCHONDRIA [J].

MIRONOVA, GD ;

SIROTA, TV ;

PRONEVICH, LA ;

TROFIMENKO, NV ;

MIRONOV, GP ;

GRIGORJEV, PA ;

KONDRASHOVA, MN .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1982, 14 (04) :213-225

← 1 2 3 4 →