A photoaffinity probe for 5-hydroxyeicosanoid dehydrogenase suitable for radioiodination

被引：13

作者：

Kim, SJ

Adiyaman, Y

Saha, G

Powell, WS

Rokach, J

机构：

[1] Florida Inst Technol, Claude Pepper Inst, Melbourne, FL 32901 USA

[2] Florida Inst Technol, Dept Chem, Melbourne, FL 32901 USA

[3] McGill Univ, Dept Med, Meakins Christie Labs, Montreal, PQ H2X 2P2, Canada

来源：

TETRAHEDRON LETTERS | 2001年 / 42卷 / 27期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

5-HETE; 5-oxo-ETE; omega-functionalzed-5-HETE; 5h-dh;

D O I：

10.1016/S0040-4039(01)00699-2

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

5-Hydroxy eicosanoid dehydrogenase (5h-dh) is a key enzyme responsible for the biosynthesis of 5-oxo-ETE, a potent eosinophil chemoattractant. To facilitate the identification and characterization of jh-dh we have synthesized a photoaffinity ligand 7, designed to bind to the enzyme, and shown it to be an excellent substrate for Sh-dh. We have also synthesized a precursor 34, containing the photoaffinity label and a trimethyl tin group that can readily be displaced by iodide and will be suitable for radioiodination with I-125. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：4445 / 4448

页数：4

共 13 条

[1]

Demin PM, 1997, MENDELEEV COMMUN, P90

[2] 12-OXO-LTB(4), A KEY PIVOTAL INTERMEDIATE IN LTB(4) METABOLISM [J].